Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,090	T→C	N153S (AAC→AGC)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,090	0	T	C	100.0%	75.5 / NA	22	N153S (AAC→AGC)	dacD	D‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

ATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATC  >  NC_000913/2082014‑2082166                                                                             |                                                                             aTGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCtcat                                                                          >  2:378954/1‑81 (MQ=255)   gCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCtcatca                                                                        >  2:378963/1‑81 (MQ=255)       gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCAtt                                                                      <  1:378924/79‑1 (MQ=255)       gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTc                                                                    <  1:378925/81‑1 (MQ=255)              gCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAAc                                                             >  1:378958/1‑81 (MQ=255)               cATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACt                                                            <  2:378926/81‑1 (MQ=255)                   cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCg                                                        >  2:378965/1‑81 (MQ=255)                   cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCg                                                        >  2:378956/1‑81 (MQ=255)                      aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTg                                                     <  2:378927/81‑1 (MQ=255)                       ccATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGc                                                    >  2:378962/1‑81 (MQ=255)                         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGccc                                                  <  2:378928/81‑1 (MQ=255)                         aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGccc                                                  <  1:378929/81‑1 (MQ=255)                           gCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCAc                                                >  1:378966/1‑81 (MQ=255)                            cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCAcc                                               <  2:378930/81‑1 (MQ=255)                                 tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCa                                           <  1:378931/80‑1 (MQ=255)                                     aaaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAAtata                                      <  2:378932/81‑1 (MQ=255)                                      aaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAg                                     <  2:378933/81‑1 (MQ=255)                                        aTGCATATCCTTGAGATGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGt                                    >  1:378960/1‑80 (MQ=255)                                                    gagaTGCAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCaa                        >  2:378973/1‑80 (MQ=255)                                                          cAGCTTCTCGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGc                 <  1:378934/81‑1 (MQ=255)                                                               tctcGGCATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCAt            >  2:378967/1‑81 (MQ=255)                                                                     cATAGTTGCTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTTATTTCCg       >  1:378990/1‑80 (MQ=255)                                                                          ttgctCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATc  >  1:378978/1‑80 (MQ=255)                                                                             |                                                                             ATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAGCAACACAAGCGTCATTTCCGGAATC  >  NC_000913/2082014‑2082166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑