Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	236,552	C→A	H162Q (CAC→CAA)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	236,552	0	C	A	100.0%	93.4 / NA	26	H162Q (CAC→CAA)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/12);  total (14/12)

CCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTG  >  NC_000913/236481‑236628                                                                        |                                                                             cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCAtt                                                                      <  1:52074/80‑1 (MQ=255) cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCAtt                                                                      <  2:52073/80‑1 (MQ=255)   cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACt                                                                   <  1:52075/81‑1 (MQ=255)   cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACt                                                                   <  2:52077/81‑1 (MQ=255)   cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACt                                                                   <  2:52076/81‑1 (MQ=255)     gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTc                                                                  >  2:52121/1‑80 (MQ=255)      tAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGa                                                                >  2:52114/1‑81 (MQ=255)        aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATg                                                              >  1:52119/1‑81 (MQ=255)        aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATg                                                              >  1:52125/1‑81 (MQ=255)           gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGccc                                                           <  1:52078/81‑1 (MQ=255)             aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAg                                                         <  2:52079/81‑1 (MQ=255)               cAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGAt                                                       <  2:52080/81‑1 (MQ=255)                 gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATcc                                                     >  1:52118/1‑81 (MQ=255)                  ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCt                                                    >  2:52123/1‑81 (MQ=255)                     cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGc                                                 >  2:52122/1‑81 (MQ=255)                          cGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGaa                                             <  2:52081/80‑1 (MQ=255)                             tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                          >  1:52131/1‑80 (MQ=255)                                         aCGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGa                             >  2:725398/1‑81 (MQ=255)                                          cGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGa                             <  1:52083/80‑1 (MQ=255)                                          cGAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGa                             <  1:52082/80‑1 (MQ=255)                                           gAAATAGATAACAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc                           >  1:52126/1‑81 (MQ=255)                                                    aaCAGTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGTGGAAGTTTATCTGGCGATGACCGGTGGTCa                   >  2:52140/1‑80 (MQ=255)                                                        gTAAACGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt              <  1:52084/81‑1 (MQ=255)                                                          aaaCGAACGCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc             >  1:52139/1‑80 (MQ=255)                                                                  gCTGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt    >  2:52148/1‑81 (MQ=255)                                                                    tGCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg  >  1:52130/1‑81 (MQ=255)                                                                        |                                                                             CCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTG  >  NC_000913/236481‑236628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑