Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,101,774	T→C	intergenic (‑30/+121)	wbbL ← / ← insH‑7	interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,101,774	0	T	C	100.0%	46.2 / NA	15	intergenic (‑30/+121)	wbbL/insH‑7	interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

TCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT  >  NC_000913/2101697‑2101825                                                                              |                                                    tCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAtcctt                                                  <  1:382491/81‑1 (MQ=255)  caacaaCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAtccttc                                                 >  2:382518/1‑81 (MQ=255)           tATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGAc                                        <  2:382492/81‑1 (MQ=255)            aTCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACt                                       >  2:382516/1‑81 (MQ=255)             tCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTc                                      <  1:382493/81‑1 (MQ=255)                gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGt                                   >  1:382517/1‑81 (MQ=255)                 aaTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtc                                  <  1:382494/81‑1 (MQ=255)                    aGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtcatt                               >  2:382533/1‑81 (MQ=255)                     gATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtcattt                              <  2:382495/81‑1 (MQ=255)                     gATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtcatt                               <  1:382496/80‑1 (MQ=255)                       tACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtcatttca                            >  1:382497/1‑81 (MQ=255)                                     aTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg              <  2:382498/81‑1 (MQ=255)                                          tGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc          >  1:382530/1‑80 (MQ=255)                                           gTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa        >  2:382522/1‑81 (MQ=255)                                               tattaAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt     <  2:382499/80‑1 (MQ=21)                                                attaAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt   >  1:382523/1‑81 (MQ=17)                                                 ttaAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt  >  2:382514/1‑81 (MQ=14)                                                                              |                                                    TCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT  >  NC_000913/2101697‑2101825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑