Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,170	T→C	pseudogene (220/446 nt)	wbbL ←	interrupted rhamnosyltransferase WbbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,170	0	T	C	100.0%	45.6 / NA	14	pseudogene (220/446 nt)	wbbL	interrupted rhamnosyltransferase WbbL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

AAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAA  >  NC_000913/2103094‑2103246                                                                             |                                                                             aaaTCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACa                                                                          >  1:382770/1‑81 (MQ=255)   atcatcATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATa                                                                        <  2:382746/81‑1 (MQ=255)      atcatGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGc                                                                     >  1:382764/1‑81 (MQ=255)       tcatGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGcc                                                                    <  1:382747/81‑1 (MQ=255)         atGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCAc                                                                  >  1:382752/1‑81 (MQ=255)          tGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCAcc                                                                 <  1:382748/81‑1 (MQ=255)                          cGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCACCGCAAtattattattat                                                 >  2:382762/1‑81 (MQ=255)                          cGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCACCGCAAtattattattat                                                 >  2:382765/1‑81 (MQ=255)                                    aaaCAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGcc                                       <  2:382749/81‑1 (MQ=255)                                       cAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGta                                    <  1:382750/81‑1 (MQ=255)                                                     catcTGCGGGTCTATATTTTTCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTat                      >  2:592971/1‑80 (MQ=255)                                                           cGGGTCTATATTTTTCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAata                    <  1:382751/77‑1 (MQ=255)                                                                      ttttCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCa     >  1:382769/1‑81 (MQ=255)                                                                         tCCTCTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATaa  >  2:382773/1‑81 (MQ=255)                                                                             |                                                                             AAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAA  >  NC_000913/2103094‑2103246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑