Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,777	T→C	noncoding (132/136 nt)	sibB →	small RNA SibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,777	0	T	C	100.0%	96.0 / NA	27	noncoding (132/136 nt)	sibB	small RNA SibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

TATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGA  >  NC_000913/2153699‑2153854                                                                               |                                                                              tATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCctt                                                                             >  2:391420/1‑81 (MQ=255)  aTGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTc                                                                            >  2:391409/1‑81 (MQ=255)   tGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTc                                                                            <  1:391374/80‑1 (MQ=255)              accacGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGt                                                                >  2:391424/1‑81 (MQ=255)              accacGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGt                                                                >  1:391402/1‑81 (MQ=255)                  cGATGATGAGTAGCTTCATCAAGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCg                                                            <  2:391375/81‑1 (MQ=255)                   gatgatGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGc                                                           >  1:391418/1‑81 (MQ=255)                    atgatgAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCt                                                          >  1:391412/1‑81 (MQ=255)                         gaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAt                                                     >  1:391401/1‑81 (MQ=255)                             aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                 >  2:391406/1‑81 (MQ=255)                             aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                 >  1:391415/1‑81 (MQ=255)                                   atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTa                                           <  2:391376/81‑1 (MQ=255)                                          cccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCtt                                    >  1:391413/1‑81 (MQ=255)                                               tCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGc                                >  1:391422/1‑80 (MQ=255)                                                ccTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGcc                               <  1:391377/80‑1 (MQ=255)                                                    atttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGc                           >  2:391416/1‑80 (MQ=255)                                                     tttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                         <  1:391379/81‑1 (MQ=255)                                                     tttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCaa                         <  1:391378/81‑1 (MQ=255)                                                      ttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                        >  1:391417/1‑81 (MQ=255)                                                            ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTc                   >  2:391410/1‑80 (MQ=255)                                                            ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                  <  1:391380/81‑1 (MQ=255)                                                            ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                  <  2:391381/81‑1 (MQ=255)                                                            ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCCTACCTCCTTTCGCCATGCAAGCAGTc                   >  1:391430/1‑80 (MQ=255)                                                               cTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATc               <  1:391382/81‑1 (MQ=255)                                                                      gggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc         <  2:391383/80‑1 (MQ=255)                                                                       ggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGGAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcgc       <  2:889491/81‑1 (MQ=255)                                                                           gggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAg   <  2:391384/81‑1 (MQ=255)                                                                            ggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGa  <  2:391385/81‑1 (MQ=255)                                                                               |                                                                              TATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAGA  >  NC_000913/2153699‑2153854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑