Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,945	T→C	V187A (GTC→GCC)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,945	0	T	C	100.0%	121.7 / NA	34	V187A (GTC→GCC)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/11);  total (23/11)

CTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGC  >  NC_000913/2158869‑2159017                                                                             |                                                                         cTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCggg                                                                      >  1:392559/1‑81 (MQ=255)  tGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGc                                                                     >  1:392528/1‑81 (MQ=255)         gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATcc                                                              >  1:392557/1‑81 (MQ=255)                  gCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCt                                                     <  1:392494/81‑1 (MQ=255)                  gCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCt                                                     >  2:392541/1‑81 (MQ=255)                  gCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCt                                                     <  2:392495/81‑1 (MQ=255)                    aaaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGt                                                   >  2:392534/1‑81 (MQ=255)                    aaaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGt                                                   >  1:392554/1‑81 (MQ=255)                     aaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGtt                                                  >  1:392561/1‑81 (MQ=255)                       tCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGttt                                                 >  1:392535/1‑80 (MQ=255)                            ggTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAg                                           >  1:392560/1‑81 (MQ=255)                             gtgtTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCTGCAGGCGCTGTTTAATCAgg                                          >  2:392558/1‑81 (MQ=255)                                ttGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCg                                       >  2:392569/1‑81 (MQ=255)                                   gTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCgtg                                     <  1:392496/80‑1 (MQ=255)                                    tGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTc                                   <  1:392497/81‑1 (MQ=255)                                     gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCg                                  >  1:392538/1‑81 (MQ=255)                                     gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCg                                  >  2:392555/1‑81 (MQ=255)                                        gtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTg                               >  1:392552/1‑81 (MQ=255)                                         tcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTgg                              <  1:392498/81‑1 (MQ=255)                                           gatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGgac                            >  2:392564/1‑81 (MQ=255)                                           gatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGga                             <  1:392499/80‑1 (MQ=255)                                            atgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGgacg                           <  2:392500/81‑1 (MQ=255)                                              gtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGgacgac                         >  2:392566/1‑81 (MQ=255)                                               tcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGacgacg                        >  2:392547/1‑81 (MQ=255)                                                cgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGacgacg                        >  1:392539/1‑80 (MQ=255)                                                 ggAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGt                       <  1:392501/80‑1 (MQ=255)                                                  gAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTAc                     <  1:392502/81‑1 (MQ=255)                                                   aGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACg                    <  2:392503/81‑1 (MQ=255)                                                      cAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAc                 >  2:392548/1‑81 (MQ=255)                                                        gCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccg               <  2:392504/81‑1 (MQ=255)                                                         cTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccgc              >  2:392551/1‑81 (MQ=255)                                                           cACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccgccg            >  2:392553/1‑81 (MQ=255)                                                            aCTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGt           >  1:392562/1‑81 (MQ=255)                                                                      gTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGc  >  1:392563/1‑80 (MQ=255)                                                                             |                                                                         CTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGC  >  NC_000913/2158869‑2159017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑