Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,770	C→A	A4A (GCG→GCT)	yegW ←	putative DNA‑binding transcriptional regulator YegW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,770	0	C	A	100.0%	112.5 / NA	32	A4A (GCG→GCT)	yegW	putative DNA‑binding transcriptional regulator YegW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/21);  total (11/21)

GTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGCGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCT  >  NC_000913/2182690‑2182845                                                                                 |                                                                            gTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATgc                                                                             >  2:396974/1‑81 (MQ=255)     cGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCt                                                                          <  1:396938/80‑1 (MQ=255)     cGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCt                                                                          <  2:396939/80‑1 (MQ=255)        cAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGtt                                                                      >  2:396981/1‑81 (MQ=255)          aaCTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTcc                                                                    <  1:396940/81‑1 (MQ=255)            cTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCAt                                                                  <  2:396941/81‑1 (MQ=255)               aaTATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCAttt                                                                <  1:396943/80‑1 (MQ=255)               aaTATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCAtt                                                                 <  1:396942/79‑1 (MQ=255)                      aGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCttttt                                                        <  1:396945/81‑1 (MQ=255)                      aGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCttttt                                                        <  2:396944/81‑1 (MQ=255)                        cggcgTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTcc                                                      >  2:396990/1‑81 (MQ=255)                        cggcgTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTcc                                                      >  1:396978/1‑81 (MQ=255)                         ggcgTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCt                                                     >  1:396979/1‑81 (MQ=255)                            gtgtTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGcc                                                  <  1:396946/81‑1 (MQ=255)                            gtgtTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGcc                                                  >  2:396984/1‑81 (MQ=255)                              gtTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCgg                                                >  1:396986/1‑81 (MQ=255)                                 aTCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCgggg                                              <  1:396947/80‑1 (MQ=255)                                    cgccgcTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGt                                           <  2:396948/80‑1 (MQ=255)                                            aaaTACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCtggtgg                                    <  1:396949/79‑1 (MQ=255)                                              aTACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGt                                <  1:396950/81‑1 (MQ=255)                                                aCGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGta                               >  1:396977/1‑80 (MQ=255)                                                aCGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGta                               >  1:396976/1‑80 (MQ=255)                                                   cTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATc                           <  2:396951/81‑1 (MQ=255)                                                      gTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCAc                         <  2:396952/80‑1 (MQ=255)                                                      gTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACt                        <  1:396953/81‑1 (MQ=255)                                                      gTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACt                        >  1:396980/1‑81 (MQ=255)                                                         cAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGc                      <  2:396954/80‑1 (MQ=255)                                                         cAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGc                      <  2:396955/80‑1 (MQ=255)                                                             tGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGCGTATAGCACTGcttct                  >  1:396991/1‑80 (MQ=255)                                                              gggCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGcttctt                 <  2:396956/80‑1 (MQ=255)                                                                       gCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCg       <  2:396957/81‑1 (MQ=255)                                                                          gggTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGttt    >  1:396993/1‑81 (MQ=255)                                                                            gTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCt  <  1:396958/81‑1 (MQ=255)                                                                                 |                                                                            GTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGCGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCT  >  NC_000913/2182690‑2182845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑