Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	245,180	C→A	R209L (CGT→CTT)	yafK ←	L,D‑transpeptidase domain‑containing protein YafK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	245,180	0	C	A	100.0%	102.7 / NA	29	R209L (CGT→CTT)	yafK	L,D‑transpeptidase domain‑containing protein YafK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/14);  total (15/14)

GCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTT  >  NC_000913/245106‑245256                                                                           |                                                                             gCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGttt                                                                        <  2:53710/81‑1 (MQ=255) gCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGtt                                                                         <  2:53711/80‑1 (MQ=255)     cacaACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCt                                                                     <  2:53712/80‑1 (MQ=255)         aCTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAag                                                                >  1:53758/1‑81 (MQ=255)            tCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAagtag                                                             >  2:53760/1‑81 (MQ=255)             cGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAagta                                                              <  1:53713/79‑1 (MQ=255)               tGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCg                                                          >  1:53754/1‑81 (MQ=255)                      aCGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAAccc                                                    <  2:53714/80‑1 (MQ=255)                        ggCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGc                                                 >  1:53755/1‑81 (MQ=255)                           ttGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCtt                                               >  1:53757/1‑80 (MQ=255)                              cTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGt                                           <  1:53715/81‑1 (MQ=255)                                 aCCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTAAAAGTAGTCGTAACCCGGCTTCAGtt                                          <  1:53716/79‑1 (MQ=255)                                     cGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTccc                                    <  2:53717/81‑1 (MQ=255)                                      gTAACGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCa                                   <  1:53718/81‑1 (MQ=255)                                         aCGACCATTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGaa                                <  2:53719/81‑1 (MQ=255)                                               aTTGACCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCt                           >  2:53765/1‑80 (MQ=255)                                                   aCCACAGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTaaa                       <  2:53720/80‑1 (MQ=255)                                                      acaGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTg                   >  1:53768/1‑81 (MQ=255)                                                      acaGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTg                   <  2:53721/81‑1 (MQ=255)                                                      acaGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTg                   >  2:53756/1‑81 (MQ=255)                                                       caGAAACGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTg                   >  1:53773/1‑80 (MQ=255)                                                        aGAAACGGTTGGTGGCCTAAGGGTTTGCTCAAAGTAGTCGTAACGCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTgg                  >  2:53759/1‑80 (MQ=255)                                                           aaCGGTTGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAata              >  2:53764/1‑81 (MQ=255)                                                                ttGGTGGCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTAt         >  1:53771/1‑81 (MQ=255)                                                                 tggtggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATg        >  1:53770/1‑81 (MQ=255)                                                                  ggtggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATgg       >  1:53763/1‑81 (MQ=255)                                                                   gtggCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATgg       <  1:53722/80‑1 (MQ=255)                                                                      gCTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCt   >  1:53772/1‑81 (MQ=255)                                                                       cTTAAGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCtt  <  2:53723/81‑1 (MQ=255)                                                                           |                                                                             GCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTT  >  NC_000913/245106‑245256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑