Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,202,498	A→T	A740A (GCA→GCT)	yehI →	DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,202,498	0	A	T	100.0%	98.5 / NA	28	A740A (GCA→GCT)	yehI	DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/18);  total (10/18)

AACCGCTGGCAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAA  >  NC_000913/2202421‑2202570                                                                              |                                                                         aaCCGCTGGCAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCg                                                                        >  2:400192/1‑80 (MQ=255)    cGCTGGCAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCAtt                                                                    <  2:400151/81‑1 (MQ=255)     gCTGGCAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTc                                                                   <  2:400152/81‑1 (MQ=255)          cAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCaaaa                                                              <  1:400153/81‑1 (MQ=255)            gCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGc                                                            <  1:400154/81‑1 (MQ=255)            gCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGc                                                            >  2:400188/1‑81 (MQ=255)              gTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGccc                                                          >  2:400196/1‑81 (MQ=255)               tACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGcccc                                                         >  2:400186/1‑81 (MQ=255)                            tCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCAt                                            <  1:400155/81‑1 (MQ=255)                            tCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCAt                                            >  1:400199/1‑81 (MQ=255)                                 ccTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGc                                        >  2:400193/1‑80 (MQ=255)                                    ggAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAAt                                    >  1:400189/1‑81 (MQ=255)                                     gAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAAt                                    <  2:400156/80‑1 (MQ=255)                                       gCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAAtgtg                                 <  1:400157/81‑1 (MQ=255)                                          gTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTc                              <  2:400158/81‑1 (MQ=255)                                           ttGCTTAAACAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTcc                             <  1:400159/81‑1 (MQ=255)                                           ttGCTTAAACAGGGTCCAATGGACATTTACCCGGATCGCATTCCAAAGGCCCCTGATTTCTGGCATCCGCAAATGTGGTcc                             <  2:400160/81‑1 (MQ=255)                                                 aaaCAGGGTCCAATGGAGATTTACCCGGCTCGGATTCGAAAAGCCCCTGATTTGTGGAATAGGAAAATGTGGTCGAggagg                       <  2:939185/81‑4 (MQ=255)                                                 aaaCAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCcg                       >  1:400201/1‑81 (MQ=255)                                                  aaCAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCcgc                      >  1:400195/1‑81 (MQ=255)                                                    cAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCgcgc                    <  2:400161/81‑1 (MQ=255)                                                    cAGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCgcgc                    >  1:400194/1‑81 (MQ=255)                                                     aGGGTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGcc                   <  2:400162/81‑1 (MQ=255)                                                       ggTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCtt                 <  1:400163/81‑1 (MQ=255)                                                       ggTCCAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCtt                 <  1:400164/81‑1 (MQ=255)                                                           cAATGGACATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCa             <  1:400165/81‑1 (MQ=255)                                                                 aCATTTACCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACtaata       <  2:400166/81‑1 (MQ=255)                                                                       aCCCGGCTCGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCaa  <  1:400167/80‑1 (MQ=255)                                                                              |                                                                         AACCGCTGGCAGCGTACAGATGTATGGTCTGCCCTGGAGCAGTTGCTTAAACAGGGTCCAATGGACATTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAA  >  NC_000913/2202421‑2202570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑