Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,258	T→C	L96P (CTA→CCA)	yehK →	uncharacterized protein YehK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,258	0	T	C	100.0%	50.3 / NA	15	L96P (CTA→CCA)	yehK	uncharacterized protein YehK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/10);  total (5/10)

GATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGG  >  NC_000913/2204185‑2204313                                                                          |                                                        gaTGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAat                                                  <  1:400455/81‑1 (MQ=255)  aTGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAata                                                 <  2:400456/81‑1 (MQ=255)   tGAGTGTATT‑‑TGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAatatat                                              <  2:400457/81‑1 (MQ=255)             gatgaGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAAcc                                        >  2:400473/1‑79 (MQ=255)              atgaGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAg                                     <  1:400458/81‑1 (MQ=255)                gaGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGa                                    >  1:400472/1‑80 (MQ=255)                  gCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAAt                                 <  2:400459/81‑1 (MQ=255)                   cGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATa                                <  1:400460/81‑1 (MQ=255)                                gTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATActc                   <  1:400461/81‑1 (MQ=255)                                gTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATActc                   <  1:400462/81‑1 (MQ=255)                                 tGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATActct                  <  1:400463/81‑1 (MQ=255)                                   aaTCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATACTCTaa                <  1:400464/81‑1 (MQ=255)                                   aaTCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATACTCTa                 >  2:400476/1‑80 (MQ=255)                                             aaCTGTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCaaa      >  2:400475/1‑81 (MQ=255)                                                 gTAATAGCAATTTTTAATCGTGAACCACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTgg  >  2:400474/1‑81 (MQ=255)                                                                          |                                                        GATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGG  >  NC_000913/2204185‑2204313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑