Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,222,467	G→A	D95N (GAT→AAT)	dld →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,222,467	0	G	A	100.0%	187.4 / NA	50	D95N (GAT→AAT)	dld	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

GACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATC  >  NC_000913/2222389‑2222542                                                                               |                                                                            gACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAAt                                                                           >  2:404029/1‑81 (MQ=255)  aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg                                                                          >  1:404044/1‑81 (MQ=255)  aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg                                                                          >  2:404032/1‑81 (MQ=255)      aaTTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGt                                                                      <  1:403983/81‑1 (MQ=255)       attattCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGtt                                                                     <  2:403984/81‑1 (MQ=255)          attCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTat                                                                   <  1:403985/80‑1 (MQ=255)           ttCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatca                                                                 <  2:403986/81‑1 (MQ=255)             cTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatcat                                                                <  1:403988/80‑1 (MQ=255)             cTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatcat                                                                <  1:403987/80‑1 (MQ=255)                aTGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAg                                                             <  2:403989/80‑1 (MQ=255)                  gCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAc                                                          >  1:404037/1‑81 (MQ=255)                   cAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAcc                                                         <  1:403990/81‑1 (MQ=255)                   cAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAcc                                                         >  2:404033/1‑81 (MQ=255)                    aGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAccc                                                        >  2:404049/1‑81 (MQ=255)                      gccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTg                                                      <  2:403991/81‑1 (MQ=255)                       ccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGc                                                     >  2:404046/1‑81 (MQ=255)                        cgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCg                                                    <  1:403992/81‑1 (MQ=255)                          ccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtc                                                  <  2:403993/81‑1 (MQ=255)                            aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc                                                >  2:404030/1‑81 (MQ=255)                            aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc                                                >  1:404028/1‑81 (MQ=255)                            aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc                                                <  1:403994/81‑1 (MQ=255)                             aTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc                                                >  2:404041/1‑80 (MQ=255)                                  ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg                                          >  1:404054/1‑81 (MQ=255)                                  ggCCTAACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGCCGTTATCATCAGCACCCTGCGTCTCGACAAg                                          >  2:404067/1‑81 (MQ=255)                                   gCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGc                                         >  2:404045/1‑81 (MQ=255)                                        aCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGc                                      <  2:403996/79‑1 (MQ=255)                                        aCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGc                                      <  2:403995/79‑1 (MQ=255)                                          cGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGt                                  >  2:404061/1‑81 (MQ=255)                                           gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt                                 >  1:404058/1‑81 (MQ=255)                                            aaGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc                                >  2:404042/1‑81 (MQ=255)                                             aGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCt                               <  1:403997/81‑1 (MQ=255)                                              ggATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCtt                              <  2:403998/81‑1 (MQ=255)                                               gATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTg                             >  1:404040/1‑81 (MQ=255)                                                 tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc                           <  1:403999/81‑1 (MQ=255)                                                  cGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc                           >  2:404051/1‑80 (MQ=255)                                                   gACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCaa                         <  2:635631/81‑1 (MQ=255)                                                    aCGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAg                        <  2:404000/81‑1 (MQ=255)                                                    aCGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAg                        >  1:404043/1‑81 (MQ=255)                                                         aaaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCg                    >  1:404038/1‑80 (MQ=255)                                                            cGGTAACGATTATGATTGCAATGTCGTTATCATCAGCACCCTGCGTCCCGACAAGCTGCACGTTCTTGGCAAGGGCGaa                  >  1:404047/1‑79 (MQ=255)                                                            cGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACa                <  1:404001/81‑1 (MQ=255)                                                              gTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAgg              >  1:404050/1‑81 (MQ=255)                                                                aaCGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTg            >  2:404056/1‑81 (MQ=255)                                                                 aCGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGc           <  1:404002/81‑1 (MQ=255)                                                                   gATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTg         >  1:404066/1‑81 (MQ=255)                                                                       aTGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGcc      >  1:404065/1‑80 (MQ=255)                                                                        tGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt     <  1:404003/80‑1 (MQ=255)                                                                          aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc  >  1:404052/1‑81 (MQ=255)                                                                          aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc  <  1:404004/81‑1 (MQ=255)                                                                          aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc  >  2:404055/1‑81 (MQ=255)                                                                               |                                                                            GACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATC  >  NC_000913/2222389‑2222542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑