Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,328	G→A	G99D (GGC→GAC)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,328	0	G	A	100.0%	109.2 / NA	30	G99D (GGC→GAC)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

TGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCT  >  NC_000913/2234251‑2234403                                                                              |                                                                            tgCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGAcc                                                                           <  1:406099/80‑1 (MQ=255) tgCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCg                                                                          <  2:406100/81‑1 (MQ=255)    cAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCtt                                                                       <  2:406102/81‑1 (MQ=255)    cAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCtt                                                                       <  2:406101/81‑1 (MQ=255)     agagTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTa                                                                      >  2:406141/1‑81 (MQ=255)              ccGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGtttt                                                             <  1:406103/81‑1 (MQ=255)               cGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTg                                                            >  2:406135/1‑81 (MQ=255)                    gagaAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACt                                                       <  1:406104/81‑1 (MQ=255)                        aaTTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGAtt                                                   >  2:406148/1‑81 (MQ=255)                        aaTTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGAtt                                                   >  1:406137/1‑81 (MQ=255)                         aTTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGAttt                                                  <  1:406105/81‑1 (MQ=255)                         aTTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGAttt                                                  <  1:406106/81‑1 (MQ=255)                         aTTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGAttt                                                  <  1:406107/81‑1 (MQ=255)                                 aaaGCGGTTGTACCCGTGCCGGTGTTGATGCACCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaacaac                                          <  2:406108/81‑1 (MQ=255)                                  aaGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaacaac                                          >  1:406134/1‑80 (MQ=255)                                        ttGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCgg                                   <  1:406109/81‑1 (MQ=255)                                          gTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGaa                                 >  2:406130/1‑81 (MQ=255)                                           tACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAAt                                >  1:406145/1‑81 (MQ=255)                                               cGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGa                             >  1:406133/1‑80 (MQ=255)                                                     ggTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTAt                      <  1:406110/81‑1 (MQ=255)                                                         ttGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAg                   <  1:406111/80‑1 (MQ=255)                                                           gATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGcc                 <  1:406112/80‑1 (MQ=255)                                                            aTGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCg               >  2:406140/1‑81 (MQ=255)                                                             tGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCgg              >  1:406156/1‑81 (MQ=255)                                                               cTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTa            <  1:406113/81‑1 (MQ=255)                                                               cTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTa            >  2:406136/1‑81 (MQ=255)                                                                  ccATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACt          >  1:406153/1‑80 (MQ=255)                                                                  ccATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACt          <  2:406114/80‑1 (MQ=255)                                                                        atatCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGc   >  2:406138/1‑81 (MQ=255)                                                                         tatCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCt  >  1:406155/1‑81 (MQ=255)                                                                              |                                                                            TGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCT  >  NC_000913/2234251‑2234403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑