Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,203	T→A	Q49L (CAA→CTA)	mglB ←	D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,203	0	T	A	100.0%	110.3 / NA	31	Q49L (CAA→CTA)	mglB	D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/25);  total (6/25)

ATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAG  >  NC_000913/2240130‑2240272                                                                          |                                                                      aTCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAAt                                                                <  1:407140/81‑1 (MQ=255) aTCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAAt                                                                <  2:407139/81‑1 (MQ=255)    gTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGc                                                             >  1:407190/1‑81 (MQ=255)       cTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCtt                                                          >  1:407180/1‑81 (MQ=255)           ttGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTgcg                                                       >  2:407195/1‑80 (MQ=255)             ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTgcgc                                                      <  2:407143/79‑1 (MQ=255)             ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCa                                                     <  1:407141/80‑1 (MQ=255)             ggACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCa                                                     <  2:407142/80‑1 (MQ=255)              gACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCAc                                                    <  1:407145/80‑1 (MQ=255)              gACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACt                                                   <  2:407144/81‑1 (MQ=255)                   gTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAg                                               <  2:407146/80‑1 (MQ=255)                      aTTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACAt                                           <  1:407148/81‑1 (MQ=255)                      aTTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACAt                                           <  2:407147/81‑1 (MQ=255)                         cTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATaa                                         <  2:407149/80‑1 (MQ=255)                            agaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGtt                                     <  1:407150/81‑1 (MQ=255)                             gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa                                    <  1:407152/81‑1 (MQ=255)                             gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa                                    <  1:407151/81‑1 (MQ=255)                             gaATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTa                                    <  1:407153/81‑1 (MQ=255)                              aaTCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTAt                                   <  1:407154/81‑1 (MQ=255)                                     catcaGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATcgtcgt                             <  2:407155/80‑1 (MQ=255)                                     catcaGCAGCTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATcgtcgt                             <  2:407156/80‑1 (MQ=255)                                         agcagcTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTta                        <  1:407157/81‑1 (MQ=255)                                         agcagcTGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTta                        >  1:407193/1‑81 (MQ=255)                                               tGAACATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTATAGACATAAAGTTATCGTCGTACTTATAGAtt                  >  2:407183/1‑81 (MQ=255)                                                 aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGAtt                  <  1:407159/79‑1 (MQ=255)                                                 aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGt                <  2:407158/81‑1 (MQ=255)                                                 aaCATCTGGCGCGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGt                <  2:407160/81‑1 (MQ=255)                                                         gcgcGGCTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTaca            <  1:407161/77‑1 (MQ=255)                                                               cTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg  <  2:407162/81‑1 (MQ=255)                                                               cTTTCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg  <  2:407163/81‑1 (MQ=255)                                                                tttCGCATCTAGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAg  >  2:407191/1‑80 (MQ=255)                                                                          |                                                                      ATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACAGACATAAAGTTATCGTCGTACTTATAGATTGTTACACCAATGCGAG  >  NC_000913/2240130‑2240272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑