Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,335	A→G	T106T (ACT→ACC)	folE ←	GTP cyclohydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,335	0	A	G	100.0%	89.8 / NA	25	T106T (ACT→ACC)	folE	GTP cyclohydrolase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/15);  total (10/15)

ACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGT  >  NC_000913/2243258‑2243411                                                                              |                                                                             aCCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGt                                                                            <  1:407779/80‑1 (MQ=255) aCCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGt                                                                            >  2:407780/1‑80 (MQ=255) aCCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGt                                                                            >  1:407810/1‑80 (MQ=255) aCCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGt                                                                            >  1:407809/1‑80 (MQ=255)   cGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGat                                                                         <  1:407781/81‑1 (MQ=255)      aTCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATc                                                                      <  2:407782/81‑1 (MQ=255)      aTCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATc                                                                      >  1:407813/1‑81 (MQ=255)           tCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATcgcg                                                                   <  1:407783/79‑1 (MQ=255)           tCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATcgc                                                                    <  2:407784/78‑1 (MQ=255)            cGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCa                                                                 <  1:407785/80‑1 (MQ=255)             gggATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACg                                                               >  1:407812/1‑81 (MQ=255)                     ggCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCAt                                                       <  1:407786/81‑1 (MQ=255)                          ccGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCAt                                                  <  2:407787/81‑1 (MQ=255)                            gTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCg                                                <  1:407788/81‑1 (MQ=255)                               gCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGAcc                                             <  1:407789/81‑1 (MQ=255)                                            tGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGtttt                                <  1:407790/81‑1 (MQ=255)                                                  cAAAATGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGa                          <  1:407791/81‑1 (MQ=255)                                                   aaaaTGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAg                         <  1:407792/81‑1 (MQ=255)                                                   aaaaTGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAg                         >  2:407819/1‑81 (MQ=255)                                                    aaaTGGTGTTCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAgg                        <  1:407793/81‑1 (MQ=255)                                                             tCACAGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTc               >  2:407824/1‑81 (MQ=255)                                                               acaGGTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCgg             <  1:407794/81‑1 (MQ=255)                                                                   gTGCTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGaaa         >  1:407811/1‑81 (MQ=255)                                                                      cTGGTCAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTg      >  1:407820/1‑81 (MQ=255)                                                                           cAGGGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGt  >  1:407817/1‑80 (MQ=255)                                                                              |                                                                             ACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGT  >  NC_000913/2243258‑2243411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑