Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,248,232	T→A	N101Y (AAC→TAC)	lysP ←	lysine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,232	0	T	A	100.0%	96.0 / NA	27	N101Y (AAC→TAC)	lysP	lysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/19);  total (8/19)

CGGGAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAAC  >  NC_000913/2248155‑2248301                                                                              |                                                                      cGGGAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAcca                                                                    <  1:408763/81‑1 (MQ=255) cGGGAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAcc                                                                     <  1:408764/80‑1 (MQ=255) cGGGAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAcc                                                                     <  2:408762/80‑1 (MQ=255)  gggAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAccag                                                                   <  2:408765/81‑1 (MQ=255)   ggAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAccagc                                                                  >  2:408805/1‑81 (MQ=255)    gAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAccagcc                                                                 <  2:408766/81‑1 (MQ=255)        caccaGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTAccagcccagc                                                             >  2:408804/1‑81 (MQ=255)            aGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGcgcg                                                          >  2:408767/1‑80 (MQ=255)                cATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGc                                                      >  2:408810/1‑80 (MQ=255)                 aTGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGc                                                      <  2:408768/79‑1 (MQ=255)                 aTGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCa                                                    <  1:408769/81‑1 (MQ=255)                       agctgagctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCct                                              >  2:408812/1‑81 (MQ=255)                         ctgagctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCcttc                                            >  2:408806/1‑81 (MQ=255)                         ctgagctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCcttc                                            <  1:408770/81‑1 (MQ=255)                            agctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCcttctt                                          >  1:408811/1‑80 (MQ=255)                            agctgCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCcttctt                                          <  1:408771/80‑1 (MQ=255)                                   aCCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAg                                  <  2:408772/81‑1 (MQ=255)                                               gCGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTaa                      <  1:408773/81‑1 (MQ=255)                                                cGATAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAg                     <  1:408774/81‑1 (MQ=255)                                                   tAGTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTgg                  <  1:408775/81‑1 (MQ=255)                                                     gTCACCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGc                 >  2:408807/1‑80 (MQ=255)                                                        aCCGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCaaa              <  1:408776/80‑1 (MQ=255)                                                         ccGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACg            <  2:408777/81‑1 (MQ=255)                                                         ccGCCCAGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACg            <  2:408778/81‑1 (MQ=255)                                                               aGTTGTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCgg      <  2:408779/81‑1 (MQ=255)                                                                   gTACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGaaa   <  1:408780/80‑1 (MQ=255)                                                                    tACCAGTAGTACCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAAc  <  2:408781/80‑1 (MQ=255)                                                                              |                                                                      CGGGAACCACCAGCTCATGACCAGCTGAGCTGCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAAC  >  NC_000913/2248155‑2248301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑