Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,072	T→C	F267S (TTT→TCT)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,072	0	T	C	100.0%	92.7 / NA	26	F267S (TTT→TCT)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

GGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTC  >  NC_000913/2275996‑2276141                                                                             |                                                                      ggCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctat                                                                    <  2:413634/80‑1 (MQ=255) ggCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtcta                                                                     <  2:413633/79‑1 (MQ=255)  gCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctattt                                                                  <  1:413635/81‑1 (MQ=255)    gTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGtctatttta                                                                >  1:413669/1‑81 (MQ=255)       aGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATg                                                              >  2:413661/1‑80 (MQ=255)         cGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAg                                                           >  2:413659/1‑81 (MQ=255)           aTCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGtt                                                         <  1:413636/81‑1 (MQ=255)           aTCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGtt                                                         >  1:413663/1‑81 (MQ=255)                aGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGAt                                                     >  1:413672/1‑80 (MQ=255)                      atcCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAaccacc                                              <  1:413637/81‑1 (MQ=255)                      atcCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAaccacc                                              >  1:413673/1‑81 (MQ=255)                         cTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCt                                            >  1:413681/1‑80 (MQ=255)                             gTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACct                                       >  2:413665/1‑81 (MQ=255)                                atatGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcg                                     <  2:413638/80‑1 (MQ=255)                                 tatGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcgct                                   >  2:413683/1‑81 (MQ=255)                                  atGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcgctc                                  >  1:413679/1‑81 (MQ=255)                                   tGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACctcgctc                                  <  1:413639/80‑1 (MQ=255)                                      tGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGAtt                              >  2:413677/1‑81 (MQ=255)                                       gCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGAttt                             >  2:413666/1‑81 (MQ=255)                                         cTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTcc                           <  2:413640/81‑1 (MQ=255)                                         cTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTcc                           <  1:413641/81‑1 (MQ=255)                                            aTGCCATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCg                        >  1:413676/1‑81 (MQ=255)                                            aTGCCATGGTCGCGACCCTCACCTTTTAACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTc                         <  1:413642/80‑1 (MQ=255)                                               ccATGGTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGc                      <  1:413643/80‑1 (MQ=255)                                                    gTCGCGACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCgg                 <  1:413644/80‑1 (MQ=255)                                                       gcgACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCt              <  1:413645/80‑1 (MQ=255)                                                       gcgACCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTa             >  1:413680/1‑81 (MQ=255)                                                          aCCCTCACCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCg          >  1:413675/1‑81 (MQ=255)                                                                aCCTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCgg     <  1:413646/80‑1 (MQ=255)                                                                  cTTTTTACCGTCTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTc  <  1:413647/81‑1 (MQ=255)                                                                             |                                                                      GGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTC  >  NC_000913/2275996‑2276141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑