Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,295,698	C→T	G208D (GGC→GAC)	ccmC ←	heme trafficking system membrane protein CcmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,295,698	0	C	T	100.0%	107.1 / NA	31	G208D (GGC→GAC)	ccmC	heme trafficking system membrane protein CcmC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/8);  total (23/8)

ACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATG  >  NC_000913/2295621‑2295771                                                                              |                                                                          acggacggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCaa                                                                        <  1:417437/81‑1 (MQ=255) acggacggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCa                                                                         >  2:417460/1‑80 (MQ=255)     acggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAAt                                                                    >  2:417471/1‑81 (MQ=255)     acggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAAt                                                                    <  2:417438/81‑1 (MQ=255)      cggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATc                                                                   >  1:417474/1‑81 (MQ=255)       ggCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATc                                                                   >  2:417466/1‑80 (MQ=255)         cGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGAc                                                                >  1:417458/1‑81 (MQ=255)         cGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGAc                                                                >  2:417454/1‑81 (MQ=255)          gTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGAcc                                                               <  2:417439/81‑1 (MQ=255)                       cAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAgcggcg                                                   >  2:417488/1‑80 (MQ=255)                         aaaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAAc                                                >  2:417476/1‑81 (MQ=255)                          aaTCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACg                                               <  2:417440/81‑1 (MQ=255)                                  tACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCg                                       >  2:417515/1‑81 (MQ=255)                                  tACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCg                                       >  2:417491/1‑81 (MQ=255)                                  tACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCg                                       >  1:417478/1‑81 (MQ=255)                                   aCGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCg                                       >  1:417486/1‑80 (MQ=255)                                    cgcatccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCgg                                      <  2:417441/80‑1 (MQ=255)                                      catccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATc                                   <  1:417442/81‑1 (MQ=255)                                         ccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGa                                 >  2:417468/1‑80 (MQ=255)                                            catcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACt                             <  1:417443/81‑1 (MQ=255)                                             atcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACtt                            >  1:417475/1‑81 (MQ=255)                                             atcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACtt                            <  1:417444/81‑1 (MQ=255)                                                    tcagcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCa                     >  2:417493/1‑81 (MQ=255)                                                      agcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATc                   >  1:417481/1‑81 (MQ=255)                                                      agcgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATc                   >  1:417479/1‑81 (MQ=255)                                                        cgtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcg                 >  1:417494/1‑81 (MQ=255)                                                         gtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgc                >  1:417480/1‑81 (MQ=255)                                                         gtGGCAGACAGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCcgc                >  1:417495/1‑81 (MQ=255)                                                                  aGGAGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATccc       >  1:417485/1‑81 (MQ=255)                                                                     aGCAGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGa    >  2:417487/1‑81 (MQ=255)                                                                        aGGAAGTCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATg  >  2:417497/1‑80 (MQ=255)                                                                              |                                                                          ACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATG  >  NC_000913/2295621‑2295771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑