Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,245	C→T	S242L (TCA→TTA)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,245	0	C	T	100.0%	116.6 / NA	32	S242L (TCA→TTA)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/14);  total (18/14)

GCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCG  >  NC_000913/2356167‑2356322                                                                               |                                                                              gCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTaa                                                                             >  2:429010/1‑81 (MQ=255)     aCAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTg                                                                         >  2:429008/1‑81 (MQ=255)         gCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACa                                                                     <  2:428983/81‑1 (MQ=255)          cAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACAt                                                                    <  2:428984/81‑1 (MQ=255)            aTTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACAttt                                                                  <  2:428985/81‑1 (MQ=255)            aTTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACAttt                                                                  >  1:429037/1‑81 (MQ=255)                cGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGccc                                                              >  2:429026/1‑81 (MQ=255)                cGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGcc                                                               <  2:428986/80‑1 (MQ=255)                      tAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGcgcg                                                        <  2:428987/81‑1 (MQ=255)                         agTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGcgacg                                                     <  1:428989/81‑1 (MQ=255)                         agTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGcgacg                                                     <  1:428988/81‑1 (MQ=255)                          gTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGcgacga                                                    <  1:428990/81‑1 (MQ=255)                             gATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAAt                                                  <  2:428991/80‑1 (MQ=255)                             gATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATa                                                 >  2:429036/1‑81 (MQ=255)                             gATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATa                                                 >  2:429021/1‑81 (MQ=255)                              aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATAc                                                <  1:428992/81‑1 (MQ=255)                               tCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATAcc                                               >  2:429030/1‑81 (MQ=255)                                ccGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATAccc                                              >  2:429034/1‑81 (MQ=255)                                ccGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATAccc                                              >  1:429016/1‑81 (MQ=255)                                  gcgAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCg                                             >  2:429041/1‑80 (MQ=255)                                  gcgAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCg                                             >  1:429040/1‑80 (MQ=255)                                              gAGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATg                                >  2:429027/1‑81 (MQ=255)                                               aGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGt                               >  1:429046/1‑81 (MQ=255)                                               aGTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGt                               >  1:429055/1‑81 (MQ=255)                                                gTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTc                              >  2:429022/1‑81 (MQ=255)                                                gTAAAAGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTc                              <  1:428993/81‑1 (MQ=255)                                                     aGGCATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGacaa                         <  2:428994/81‑1 (MQ=255)                                                        cATTCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGAcaacaa                      <  2:428995/81‑1 (MQ=255)                                                           tCCGGTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAgg                   >  1:429029/1‑81 (MQ=255)                                                              ggTGATTGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCtt                >  1:429031/1‑81 (MQ=255)                                                                    tGCCACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGa          >  2:429033/1‑81 (MQ=255)                                                                      ccACCTCCTTAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATc        <  2:428996/81‑1 (MQ=255)                                                                           tccttAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATc   >  1:429045/1‑81 (MQ=255)                                                                            ccttAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCg  >  1:465278/1‑81 (MQ=255)                                                                               |                                                                              GCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCG  >  NC_000913/2356167‑2356322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑