Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,002	C→T	E51K (GAA→AAA)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,002	0	C	T	100.0%	96.5 / NA	27	E51K (GAA→AAA)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/10);  total (17/10)

GCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCA  >  NC_000913/2359927‑2360076                                                                            |                                                                           gcATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTAc                                                                       >  2:429740/1‑81 (MQ=255)       aaaGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAAcc                                                                 >  1:429703/1‑81 (MQ=255)        aaGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAAccc                                                                >  1:429709/1‑81 (MQ=255)              cGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTAt                                                           <  1:429674/80‑1 (MQ=255)               gCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATc                                                          <  1:429675/80‑1 (MQ=255)               gCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATc                                                          <  1:429677/80‑1 (MQ=255)               gCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCa                                                         <  2:429676/81‑1 (MQ=255)                  ggATCACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATc                                                      >  2:429720/1‑81 (MQ=255)                  ggAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATc                                                      >  2:429700/1‑81 (MQ=255)                       acCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTgg                                                   >  1:429714/1‑79 (MQ=255)                       acCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTgg                                                   >  1:429725/1‑79 (MQ=255)                             aaaCGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTg                                           <  1:429678/81‑1 (MQ=255)                             aaaCGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTg                                           >  2:429706/1‑81 (MQ=255)                                        ccACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAc                                <  2:429679/81‑1 (MQ=255)                                         cacaAAGAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAcc                               >  1:429708/1‑81 (MQ=255)                                               gAAAATGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATAtt                         >  2:429721/1‑81 (MQ=255)                                                aaaaTGCCTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTc                        <  2:429680/81‑1 (MQ=255)                                                      ccTGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACgg                  >  1:429728/1‑81 (MQ=255)                                                        tGCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTc                >  1:429716/1‑81 (MQ=255)                                                         gCTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTc                >  1:429712/1‑80 (MQ=255)                                                          cTCCCAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCga              <  1:429681/81‑1 (MQ=255)                                                             ccAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCgaga            <  2:429682/80‑1 (MQ=255)                                                             ccAGCGCATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCgag             <  1:429683/79‑1 (MQ=255)                                                                 cgcATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGc       >  1:429715/1‑81 (MQ=255)                                                                   cATAAGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGc       >  1:429717/1‑79 (MQ=255)                                                                      aaGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGca  >  2:429719/1‑81 (MQ=255)                                                                      aaGCTTTATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGca  >  1:429707/1‑81 (MQ=255)                                                                            |                                                                           GCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAACGGTCGAGAAACGCCAGCA  >  NC_000913/2359927‑2360076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑