Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,374,272	T→C	L244L (TTA→TTG)	menE ←	o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,374,272	0	T	C	100.0%	102.1 / NA	29	L244L (TTA→TTG)	menE	o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

CCATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGC  >  NC_000913/2374195‑2374343                                                                              |                                                                        ccATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAac                                                                      <  2:432268/81‑1 (MQ=255)     aGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCg                                                                  >  2:432318/1‑81 (MQ=255)      gCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGc                                                                 <  2:432269/81‑1 (MQ=255)         gCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                              <  1:432270/81‑1 (MQ=255)          cAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                              >  1:432333/1‑80 (MQ=255)           aaaaGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt                                                              >  2:432299/1‑79 (MQ=255)            aaaGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAg                                                           >  2:432308/1‑81 (MQ=255)             aaGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAgg                                                          >  2:432316/1‑81 (MQ=255)                 aaCGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGaaa                                                      >  1:432322/1‑81 (MQ=255)                  aCGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAAc                                                     <  1:432271/81‑1 (MQ=255)                   cGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACg                                                    <  1:432272/81‑1 (MQ=255)                    gAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACg                                                    >  1:432313/1‑80 (MQ=255)                     aaTCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGa                                                  <  1:432273/81‑1 (MQ=255)                     aaTCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGa                                                  <  2:432274/81‑1 (MQ=255)                      aTCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGaa                                                 >  1:432328/1‑81 (MQ=255)                        ccccTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAAc                                                >  2:869359/1‑80 (MQ=255)                             gCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGt                                          >  2:432301/1‑81 (MQ=255)                              cTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGtt                                         <  1:432275/81‑1 (MQ=255)                               tCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGtt                                         >  2:432331/1‑80 (MQ=255)                                 gcgcgcCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAAc                                      >  2:432324/1‑81 (MQ=255)                                            ccGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGc                            >  2:432314/1‑80 (MQ=255)                                                 aaCTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGt                      >  2:432325/1‑81 (MQ=255)                                                  aCTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGtt                     <  1:432276/81‑1 (MQ=255)                                                     cGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTtgtg                  >  2:432327/1‑81 (MQ=255)                                                       aCCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGtt                <  1:432277/81‑1 (MQ=255)                                                            gATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCAc           <  2:432278/81‑1 (MQ=255)                                                                gccgcgccgcCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGt       <  1:432279/81‑1 (MQ=255)                                                                 ccgcgccgcCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGt       >  1:432323/1‑80 (MQ=255)                                                                     gccgccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGc  <  2:432280/81‑1 (MQ=255)                                                                              |                                                                        CCATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGC  >  NC_000913/2374195‑2374343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑