Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,206	A→T	V47E (GTG→GAG)	nuoK ←	NADH:quinone oxidoreductase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,206	0	A	T	100.0%	132.0 / NA	37	V47E (GTG→GAG)	nuoK	NADH:quinone oxidoreductase subunit K
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/22);  total (15/22)

CCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAG  >  NC_000913/2395135‑2395282                                                                        |                                                                             ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGcc                                                                      <  1:436128/80‑1 (MQ=255) ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGc                                                                       <  2:436130/79‑1 (MQ=255) ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCa                                                                     <  2:436129/81‑1 (MQ=255) ccgccgCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCa                                                                     <  2:436127/81‑1 (MQ=255)  cgccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAg                                                                    >  2:436167/1‑81 (MQ=255)   gccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgc                                                                   <  2:436132/81‑1 (MQ=255)   gccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgc                                                                   <  2:436131/81‑1 (MQ=255)     cgcgAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAgcgc                                                                 >  1:436179/1‑81 (MQ=255)      gcgAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg                                                                <  1:436133/81‑1 (MQ=255)        gAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg                                                                >  1:436182/1‑79 (MQ=255)        gAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGcgcg                                                                >  1:436186/1‑79 (MQ=255)          ggCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAgg                                                            <  1:436134/81‑1 (MQ=255)           gCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGc                                                           <  2:436135/81‑1 (MQ=255)               aTGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGtt                                                        >  2:436170/1‑80 (MQ=255)                  gCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAAt                                                     >  1:436172/1‑80 (MQ=255)                  gCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATc                                                    <  2:436136/81‑1 (MQ=255)                   cGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCa                                                   <  1:436137/81‑1 (MQ=255)                         tGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGAttt                                             <  2:436138/81‑1 (MQ=255)                           tACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTc                                            <  1:436139/80‑1 (MQ=255)                               tCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAc                                       <  2:436140/81‑1 (MQ=255)                                cACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAc                                       >  1:436183/1‑80 (MQ=255)                                 aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAcc                                      >  1:436190/1‑80 (MQ=255)                                 aCCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGAcc                                      <  2:436141/80‑1 (MQ=255)                                       ccGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAAc                               <  2:436142/81‑1 (MQ=255)                                         gTCGGTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACa                              >  1:436181/1‑80 (MQ=255)                                            ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaaa                          >  1:436184/1‑81 (MQ=255)                                            ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaa                           <  1:436144/80‑1 (MQ=255)                                            ggTCTGGCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATaa                           <  2:436143/80‑1 (MQ=255)                                                  gCCCCAGTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAAcagca                     <  2:436145/80‑1 (MQ=255)                                                        gTAGCTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCg              <  1:436146/81‑1 (MQ=255)                                                            cTTCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACg           >  1:436178/1‑80 (MQ=255)                                                             ttCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGa         >  2:436185/1‑81 (MQ=255)                                                              tCCGGCGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGa         >  2:436192/1‑80 (MQ=255)                                                                   cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAc     >  1:436199/1‑79 (MQ=255)                                                                   cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAAc     >  2:436189/1‑79 (MQ=255)                                                                   cGACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCa   <  2:436147/81‑1 (MQ=255)                                                                    gACCTCGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAg  <  1:436148/81‑1 (MQ=255)                                                                        |                                                                             CCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAG  >  NC_000913/2395135‑2395282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑