Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,399,514	T→C	K218E (AAA→GAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,399,514	0	T	C	100.0%	147.2 / NA	40	K218E (AAA→GAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/23);  total (17/23)

GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATT  >  NC_000913/2399437‑2399591                                                                              |                                                                              gTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACg                                                                            <  1:437140/81‑1 (MQ=255)    aCGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGtt                                                                         >  2:437190/1‑81 (MQ=255)    aCGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGtt                                                                         >  1:437191/1‑81 (MQ=255)      gTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTg                                                                        >  2:437199/1‑80 (MQ=255)        tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa                                                                     >  2:437201/1‑81 (MQ=255)        tCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa                                                                     >  1:437213/1‑81 (MQ=255)         cACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTaa                                                                     <  2:437141/80‑1 (MQ=255)          aCCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACg                                                                   <  1:437143/81‑1 (MQ=255)          aCCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACg                                                                   <  2:437142/81‑1 (MQ=255)             ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc                                                                >  2:437198/1‑81 (MQ=255)             ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc                                                                >  1:437204/1‑81 (MQ=255)             ggggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTc                                                                <  2:437144/81‑1 (MQ=255)               ggCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCgg                                                              <  1:437145/81‑1 (MQ=255)                 cTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAg                                                            >  2:437194/1‑81 (MQ=255)                    aTGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGt                                                           <  2:437146/79‑1 (MQ=255)                     tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg                                                        <  1:437148/81‑1 (MQ=255)                     tGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCg                                                        <  2:437147/81‑1 (MQ=255)                          cAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTg                                                   >  2:437207/1‑81 (MQ=255)                            gCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTc                                                 <  1:437149/81‑1 (MQ=255)                               gATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGt                                              <  1:437150/81‑1 (MQ=255)                                aTGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTa                                             <  2:437151/81‑1 (MQ=255)                                    aaCATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATa                                         <  1:437152/81‑1 (MQ=255)                                     aCATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATAc                                        <  2:437153/81‑1 (MQ=255)                                         tGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCg                                    >  1:437210/1‑81 (MQ=255)                                           cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCgg                                   <  2:437155/80‑1 (MQ=255)                                           cTGGCAGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGt                                  <  1:437154/81‑1 (MQ=255)                                                aGATGCTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGc                             <  1:437156/81‑1 (MQ=255)                                                     cTCGGCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAAtt                        <  1:437157/81‑1 (MQ=255)                                                        ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc                      <  1:437158/80‑1 (MQ=255)                                                        ggCGCAAACTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTc                      >  1:437200/1‑80 (MQ=255)                                                             aaaCTGCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAg                >  1:437208/1‑81 (MQ=255)                                                                  gCATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAc           >  2:437203/1‑81 (MQ=255)                                                                   cATATCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTAcc          >  2:437220/1‑81 (MQ=255)                                                                     tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg        <  2:437160/81‑1 (MQ=255)                                                                     tatCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCgg        <  1:437159/81‑1 (MQ=255)                                                                       tCCCATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGa       >  2:437209/1‑80 (MQ=255)                                                                        cccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaa      >  1:437205/1‑80 (MQ=255)                                                                        cccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaa      >  2:437222/1‑80 (MQ=255)                                                                         ccATTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGaaaa    <  1:437161/81‑1 (MQ=255)                                                                           aTTCACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAAtt  <  1:437162/81‑1 (MQ=255)                                                                              |                                                                              GTAACGTTCACCGGGGCTGATGTTACAGCCGATGGAACATTGCTGGCAGATGCTCGGCGCAAACTGCATATCCCATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATT  >  NC_000913/2399437‑2399591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑