Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,400	G→T	S130* (TCG→TAG)	hisJ ←	histidine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,400	0	G	T	100.0%	77.9 / NA	22	S130* (TCG→TAG)	hisJ	histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/14);  total (8/14)

CTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAG  >  NC_000913/2426323‑2426477                                                                              |                                                                              cTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGctact                                                                            >  2:442402/1‑81 (MQ=255)    ttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGctactcg                                                                          <  1:442368/80‑1 (MQ=255)    ttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGctactc                                                                           <  2:442367/79‑1 (MQ=255)      ggTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGctactcgac                                                                        <  2:442370/80‑1 (MQ=255)      ggTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGctactcgac                                                                        <  2:442369/80‑1 (MQ=255)       gTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTg                                                                      <  1:442371/81‑1 (MQ=255)        tGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGt                                                                     <  1:442372/81‑1 (MQ=255)                    aTTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGt                                                          >  2:442411/1‑80 (MQ=255)                         cGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAAt                                                    <  1:442373/81‑1 (MQ=255)                           aaCGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAAttt                                                  <  1:442374/81‑1 (MQ=255)                                          gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCaaa                                   >  2:442407/1‑81 (MQ=255)                                          gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCaa                                    <  1:477963/80‑1 (MQ=255)                                            gCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACg                                 <  2:442375/81‑1 (MQ=255)                                             cccTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACg                                 <  2:442376/80‑1 (MQ=255)                                               cTGCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACgag                               >  2:442415/1‑80 (MQ=255)                                                 gCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAAt                            >  1:442428/1‑81 (MQ=255)                                                 gCAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAAt                            >  1:442414/1‑81 (MQ=255)                                                  cAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGaa                             <  1:442379/79‑1 (MQ=255)                                                  cAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAAt                            <  2:442377/80‑1 (MQ=255)                                                  cAATACGCCTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAAt                            <  2:442378/80‑1 (MQ=255)                                                          cTACCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGt                   <  1:442380/81‑1 (MQ=255)                                                            aCCCGTTTGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTa                  >  2:442412/1‑80 (MQ=255)                                                             cccGTTTGCCTTTCAGCTACTCGACTGTCGACTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTAc                 <  2:442381/80‑1 (MQ=255)                                                                 tttGCCTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGttt            <  2:442382/81‑1 (MQ=255)                                                                      cTTTCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCgg       >  2:442433/1‑81 (MQ=255)                                                                         tCAGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTg     <  1:442383/80‑1 (MQ=255)                                                                           aGCTACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAg  >  2:442417/1‑81 (MQ=255)                                                                              |                                                                              CTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAG  >  NC_000913/2426323‑2426477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑