Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,346	T→A	K42* (AAG→TAG)	fadI ←	3‑ketoacyl‑CoA thiolase FadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,346	0	T	A	100.0%	114.3 / NA	32	K42* (AAG→TAG)	fadI	3‑ketoacyl‑CoA thiolase FadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/20);  total (12/20)

CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATGG  >  NC_000913/2460268‑2460423                                                                               |                                                                              cGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAcc                                                                             >  2:449158/1‑81 (MQ=255) cGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTAcc                                                                             >  1:449180/1‑81 (MQ=255)   aCCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCt                                                                           <  2:449136/81‑1 (MQ=255)    ccTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTa                                                                          <  1:449137/81‑1 (MQ=255)        aCCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATc                                                                      <  2:449138/81‑1 (MQ=255)          cAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCaa                                                                    <  1:449139/81‑1 (MQ=255)           aaaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAc                                                                   <  2:449141/81‑1 (MQ=255)           aaaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAc                                                                   <  2:449140/81‑1 (MQ=255)            aaGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAAcc                                                                  <  1:449142/81‑1 (MQ=255)                           aTCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGa                                                   <  1:449143/81‑1 (MQ=255)                                  cggcggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCg                                            >  2:449196/1‑81 (MQ=255)                                     cggGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCg                                         >  2:449189/1‑81 (MQ=255)                                       gggATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAGATCAACCGCGGGAATGCCATGAAAAGCCGTCGcc                                       <  1:449144/81‑1 (MQ=255)                                       gggATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGcc                                       <  1:449145/81‑1 (MQ=255)                                          aTCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGa                                    >  1:449186/1‑81 (MQ=255)                                            ctcGCTACGTGCCAGCAGATCGCCTACCACCGTCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGAc                                   <  2:449146/80‑1 (MQ=255)                                               gCTGCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACg                                  <  2:449147/78‑1 (MQ=255)                                                  gCGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCaa                             >  2:449197/1‑80 (MQ=255)                                                   cGTGCCAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCaaa                            <  2:449148/80‑1 (MQ=255)                                                       ccAGCAGTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGc                        <  2:449149/80‑1 (MQ=255)                                                        cagcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgt                      <  2:449150/81‑1 (MQ=255)                                                        cagcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgt                      <  2:449151/81‑1 (MQ=255)                                                         agcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgta                     >  2:449191/1‑81 (MQ=255)                                                         agcagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgta                     >  2:449193/1‑81 (MQ=255)                                                           cagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtacg                   >  1:449199/1‑81 (MQ=255)                                                           cagTTCGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGcgtacg                   <  1:449152/81‑1 (MQ=255)                                                                cGCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc              >  1:449190/1‑81 (MQ=255)                                                                 gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAcc             <  1:449153/81‑1 (MQ=255)                                                                 gCCTACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAAc              <  1:449154/80‑1 (MQ=255)                                                                    tACCACCATCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGc           >  2:449192/1‑80 (MQ=255)                                                                       caccaTCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAAc       <  1:449155/81‑1 (MQ=255)                                                                            tCTACCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATgg  >  1:449194/1‑81 (MQ=255)                                                                               |                                                                              CGACCTGACCAAAGACCAGTTGTTCAATCACTTCGGCGGGGATCTCGCTGCGTGCCAGCAGTTCGCCTACCACCATCTTCCCTAAATCAACCGCGGGAATGCCATGAAAAGCCGTCGCCTGACGGGCAAAAGGCGTACGTAAACCGCTAACAATGG  >  NC_000913/2460268‑2460423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑