Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,461,596	C→T	N97N (AAC→AAT)	fadL →	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,461,596	0	C	T	100.0%	89.9 / NA	25	N97N (AAC→AAT)	fadL	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

CGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAACATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGGCGCTTC  >  NC_000913/2461520‑2461670                                                                             |                                                                           cGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATc                                                                         <  1:449311/80‑1 (MQ=255) cGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATc                                                                         <  1:449310/80‑1 (MQ=255)       cGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCt                                                                   <  2:449312/80‑1 (MQ=255)        ggTTTATATTGACCCGGATGTAAATATCAGCGGAACATCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTAc                                                                 <  2:449313/81‑1 (MQ=255)         gTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACg                                                                >  2:449342/1‑81 (MQ=255)           ttATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACgg                                                               >  1:449334/1‑80 (MQ=255)              tatTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATg                                                           >  1:449331/1‑81 (MQ=255)                ttGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATggg                                                         <  1:449314/81‑1 (MQ=255)                    cccGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTcc                                                     <  2:449315/81‑1 (MQ=255)                     ccGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCg                                                    <  1:449316/81‑1 (MQ=255)                       ggATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGaa                                                  <  1:449317/81‑1 (MQ=255)                           gTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATg                                              >  2:449343/1‑81 (MQ=255)                              aaTATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCAc                                           >  1:449339/1‑81 (MQ=255)                                 atCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACttt                                        >  1:449335/1‑81 (MQ=255)                                    aGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTttgtt                                     >  2:449347/1‑81 (MQ=255)                                             tctcCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGAtt                            <  1:449318/81‑1 (MQ=255)                                             tctcCATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGAtt                            <  1:449319/81‑1 (MQ=255)                                                 cATCTGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTaa                          <  1:449320/79‑1 (MQ=255)                                                     tGGTCGTAGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGAcc                     >  1:449349/1‑80 (MQ=255)                                                            aGCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATttggt             >  2:449348/1‑81 (MQ=255)                                                             gCCTGAAAGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATttggt             <  1:449321/80‑1 (MQ=255)                                                                  aaaGCCGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGgc       >  1:449352/1‑81 (MQ=255)                                                                      ccGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGGCGCtt   <  1:449322/81‑1 (MQ=255)                                                                       cGATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGGCGCTTc  <  1:449323/81‑1 (MQ=255)                                                                        gATAATATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGGCGCTTc  >  2:449350/1‑80 (MQ=255)                                                                             |                                                                           CGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCCTGAAAGCCGATAACATCGCGCCTACGGCATGGGTTCCGAACATGCACTTTGTTGCACCGATTAACGACCAATTTGGTTGGGGCGCTTC  >  NC_000913/2461520‑2461670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑