Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,985	A→C	T229P (ACC→CCC)	yfdC →	inner membrane protein YfdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,985	0	A	C	100.0%	90.4 / NA	26	T229P (ACC→CCC)	yfdC	inner membrane protein YfdC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/14);  total (12/14)

GGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTG  >  NC_000913/2465908‑2466061                                                                              |                                                                             ggATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACaccccc                                                                            >  1:450003/1‑80 (MQ=255)        tCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGt                                                                    >  1:450029/1‑81 (MQ=255)          cTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTgg                                                                  <  2:449967/81‑1 (MQ=255)             cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGt                                                                 <  1:449970/79‑1 (MQ=255)             cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTc                                                                <  2:449969/80‑1 (MQ=255)             cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCg                                                               <  1:449968/81‑1 (MQ=255)                cGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGtt                                                            <  1:449972/81‑1 (MQ=255)                cGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGtt                                                            >  2:450008/1‑81 (MQ=255)                cGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGtt                                                            <  2:449971/81‑1 (MQ=255)                  ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCt                                                          <  1:449973/81‑1 (MQ=255)                  ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCt                                                          >  2:450013/1‑81 (MQ=255)                   gTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTg                                                         >  2:450014/1‑81 (MQ=255)                      cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGtt                                                       <  1:449974/80‑1 (MQ=255)                       ggCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTg                                                      >  1:450011/1‑80 (MQ=255)                            aGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATcc                                                >  1:450018/1‑81 (MQ=255)                                    gtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTg                                        >  1:450010/1‑81 (MQ=255)                                          atatTGATGACCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGtt                                   <  1:449975/80‑1 (MQ=255)                                                atgaCCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACgg                             >  2:450021/1‑80 (MQ=255)                                                 tgaCCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTa                           <  1:449976/81‑1 (MQ=255)                                                  gaCCTGGCTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTGTAACGGTAc                          >  2:450019/1‑81 (MQ=255)                                                         cTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                   >  1:450012/1‑81 (MQ=255)                                                         cTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                   >  1:729193/1‑81 (MQ=255)                                                         cTTATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCAc                   <  1:449977/81‑1 (MQ=255)                                                           tATTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTg                 >  2:450017/1‑81 (MQ=255)                                                            aTTGCCCTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACt                  <  2:449978/79‑1 (MQ=255)                                                                  cTGGGTGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt          <  2:449979/81‑1 (MQ=255)                                                                       tGACACCCCCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCAt     <  1:449980/81‑1 (MQ=255)                                                                          caccccCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTg  <  1:449981/81‑1 (MQ=255)                                                                              |                                                                             GGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCTG  >  NC_000913/2465908‑2466061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑