Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,234	C→A	A354A (GCG→GCT)	alaC ←	glutamate‑‑pyruvate aminotransferase AlaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,234	0	C	A	100.0%	78.1 / NA	22	A354A (GCG→GCT)	alaC	glutamate‑‑pyruvate aminotransferase AlaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/6);  total (16/6)

GCGGTTTTCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTCGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCGCCCAGA  >  NC_000913/2497156‑2497307                                                                               |                                                                          gcgGTTTTCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACcttagc                                                                         <  1:454449/81‑1 (MQ=255) gcgGTTTTCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACcttagc                                                                         >  2:454490/1‑81 (MQ=255)    gTTTTCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACcttagcttc                                                                      >  2:454473/1‑81 (MQ=255)       ttCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGt                                                                    >  1:454470/1‑80 (MQ=255)                   aaaGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGcttctt                                                       >  2:454484/1‑81 (MQ=255)                   aaaGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGcttctt                                                       >  1:454482/1‑81 (MQ=255)                        gAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCa                                                   >  2:454483/1‑80 (MQ=255)                            aTGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTc                                              <  1:454450/81‑1 (MQ=255)                            aTGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTc                                              >  1:454501/1‑81 (MQ=255)                                    cACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATc                                      >  1:454487/1‑81 (MQ=255)                                      ccATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATccc                                    <  1:454451/81‑1 (MQ=255)                                       cATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCa                                   >  1:570409/1‑81 (MQ=255)                                          aGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATgg                                >  1:454476/1‑81 (MQ=255)                                           gTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGc                               <  2:454452/81‑1 (MQ=255)                                            tCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGcc                              >  1:454477/1‑81 (MQ=255)                                                      cAATCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGtt                    >  2:454485/1‑81 (MQ=255)                                                        aTCCCCGGCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTcc                  <  1:454453/81‑1 (MQ=255)                                                              ggCGAGACACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGAtt            <  2:454454/81‑1 (MQ=255)                                                                 gagaCACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTc         >  1:454491/1‑81 (MQ=255)                                                                 gagaCACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTc         >  1:454497/1‑81 (MQ=255)                                                                 gagaCACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTc         <  2:454455/81‑1 (MQ=255)                                                                  agaCACAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCg        >  1:454494/1‑81 (MQ=255)                                                                    acacAAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCGcc      >  2:454480/1‑81 (MQ=255)                                                                      acaAACCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCGcc      >  1:454488/1‑79 (MQ=255)                                                                         aaCCTTAGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCGCCCAGa  >  1:454486/1‑80 (MQ=255)                                                                               |                                                                          GCGGTTTTCAATCAGTGCAAAGCGAACATGCGTATCACCATAGTCGCCAAAGCCAATCCCCGGCGAGACACAAACCTTCGCTTCGTTAAGGAGCTTCTTGGCAAATTCCAGCGATCCCATGGCCGCATATGGTTCCGGGATTTTCGCCCAGA  >  NC_000913/2497156‑2497307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑