Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,513,581	C→T	S180S (TCC→TCT)	nupC →	nucleoside:H(+) symporter NupC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,513,581	0	C	T	100.0%	120.0 / NA	33	S180S (TCC→TCT)	nupC	nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/10);  total (23/10)

TAAAGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCACC  >  NC_000913/2513503‑2513656                                                                               |                                                                            taAAGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTAt                                                                           <  1:457733/81‑1 (MQ=255)     gATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGtt                                                                       <  1:457734/81‑1 (MQ=255)      atatCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTg                                                                      >  2:457778/1‑81 (MQ=255)         tCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGt                                                                    <  1:457735/80‑1 (MQ=255)          ccTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTg                                                                   >  2:457759/1‑80 (MQ=255)             cGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATa                                                               >  2:457761/1‑81 (MQ=255)              ggCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATa                                                               >  2:457763/1‑80 (MQ=255)                 aaaaTCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACAt                                                            >  2:457757/1‑80 (MQ=255)                    aTCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGAcc                                                        <  1:457736/81‑1 (MQ=255)                          cGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTg                                                  >  2:457779/1‑81 (MQ=255)                             aaTCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGaa                                               >  2:457784/1‑81 (MQ=255)                                 gTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCg                                            >  1:457770/1‑80 (MQ=255)                                 gTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCg                                            >  1:457771/1‑80 (MQ=255)                                   aTGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGaaa                                         >  1:457775/1‑81 (MQ=255)                                         acCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAcgtc                                   <  1:457737/81‑1 (MQ=255)                                         acCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAcgtc                                   >  1:457765/1‑81 (MQ=255)                                         acCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATAcgtc                                   >  2:457783/1‑81 (MQ=255)                                          ccATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGGAATAcgtcg                                  >  1:457781/1‑81 (MQ=255)                                            aTGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGtt                                <  2:457738/81‑1 (MQ=255)                                             tGGCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTtg                               <  2:457739/81‑1 (MQ=255)                                               gCTGCCACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTtgct                             >  2:457769/1‑81 (MQ=255)                                                   ccACGGCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTgcg                          <  2:457740/80‑1 (MQ=255)                                                       ggCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTgg                      >  1:457790/1‑80 (MQ=255)                                                        gCAATGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTAGAACCGAAATACGTCGTTGCTGCGCTGGTa                    >  2:457773/1‑81 (MQ=255)                                                          aaTGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACt                  >  1:457786/1‑81 (MQ=255)                                                           aTGTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACt                  >  2:457776/1‑80 (MQ=255)                                                             gTCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGaa               >  2:457760/1‑81 (MQ=255)                                                              tCCACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAAc              <  1:457741/81‑1 (MQ=255)                                                                cACCGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACAt            >  1:457772/1‑81 (MQ=255)                                                                   cGTGTCGATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGtt         >  1:457785/1‑81 (MQ=255)                                                                         gATGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCAc   >  1:457774/1‑81 (MQ=255)                                                                          aTGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCAcc  <  1:457742/81‑1 (MQ=255)                                                                          aTGTCTATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCAcc  >  2:457788/1‑81 (MQ=255)                                                                               |                                                                            TAAAGATATCCTCGGCAAAATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGACCATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCACC  >  NC_000913/2513503‑2513656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑