Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,522,542	C→T	intergenic (‑65/+187)	xapR ← / ← xapB	DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,522,542	0	C	T	100.0%	24.2 / NA	8	intergenic (‑65/+187)	xapR/xapB	DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

ACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGAT  >  NC_000913/2522467‑2522619                                                                            |                                                                              aCGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGata                                                                          >  1:459379/1‑81 (MQ=255)  cgcgTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGatat                                                                         >  2:459382/1‑81 (MQ=255)        ccATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGATATCCGac                                                                    <  1:459370/80‑1 (MQ=255)        ccATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGATATCCGac                                                                    <  1:459371/80‑1 (MQ=255)           tAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGATATCCGacatac                                                                <  2:459372/81‑1 (MQ=255)           tAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGATATCCGacata                                                                 >  1:459380/1‑80 (MQ=255)                                      aTCGTATTAAATTATCATGGCATAAAGTATTTCACCACTAGATATCCGACATACATAAACACAATGGATAATATACCCgg                                      >  2:459385/1‑80 (MQ=255)                                                             aaaGTATTTCACCACTAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAgg              >  2:459383/1‑81 (MQ=255)                                                                         cactaGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGAt  <  1:459373/81‑1 (MQ=255)                                                                            |                                                                              ACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGAT  >  NC_000913/2522467‑2522619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑