Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,539,484	T→A	N34Y (AAT→TAT)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,539,484	0	T	A	100.0%	104.2 / NA	29	N34Y (AAT→TAT)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/18);  total (11/18)

TTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTA  >  NC_000913/2539407‑2539554                                                                              |                                                                       tttAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGcc                                                                     <  1:462458/81‑1 (MQ=255)    aaTTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCtt                                                                   <  1:462459/80‑1 (MQ=255)    aaTTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTc                                                                  >  2:462502/1‑81 (MQ=255)        tCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAg                                                               <  1:462461/80‑1 (MQ=255)        tCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAg                                                               <  2:462460/80‑1 (MQ=255)         ccccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtt                                                             >  1:462507/1‑81 (MQ=255)         ccccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGt                                                              <  2:462462/80‑1 (MQ=255)           ccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt                                                           <  2:462464/81‑1 (MQ=255)           ccGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt                                                           <  2:462463/81‑1 (MQ=255)            cgcgCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGtttt                                                           >  2:462496/1‑80 (MQ=255)             gcgcTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTaa                                                         >  2:462504/1‑81 (MQ=255)               gcTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAAc                                                        >  1:462503/1‑80 (MQ=255)                   ttCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACcac                                                     <  2:462465/79‑1 (MQ=255)                      cGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACtt                                                 <  1:462466/80‑1 (MQ=255)                      cGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTc                                                <  1:462467/81‑1 (MQ=255)                        ccTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCAc                                              <  2:462468/81‑1 (MQ=255)                            gacgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGcc                                          >  1:462499/1‑81 (MQ=255)                            gacgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGcc                                          <  2:462469/81‑1 (MQ=255)                             acgaTCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCg                                         <  2:462470/81‑1 (MQ=255)                                             ccGCACGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTc                         >  1:462509/1‑81 (MQ=255)                                                 aCGATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTg                     <  1:462471/81‑1 (MQ=255)                                                   gATCTTTCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGc                    >  1:462517/1‑80 (MQ=255)                                                         tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACtt               <  2:462473/79‑1 (MQ=255)                                                         tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTc              <  2:462472/80‑1 (MQ=255)                                                         tCACCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCa             >  2:462515/1‑81 (MQ=255)                                                           aCCGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCAcc           <  2:462474/81‑1 (MQ=255)                                                            ccGAACCTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCa          <  1:462475/81‑1 (MQ=255)                                                                  cTGCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCg    >  1:462518/1‑81 (MQ=255)                                                                    gCCGGGTTATAGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTa  >  2:462511/1‑81 (MQ=255)                                                                              |                                                                       TTTAATTTCCCCGCGCTTTTCCGCCTCGACGATCATCGAAAGTGCCGCACGATCTTTCACCGAACCTGCCGGGTTATTGCCTTCCAGTTTTAACCACACTTCACTGCCGTTATCCGGCCCCATTCGCTGCAACTTCACCAGAGGCGTA  >  NC_000913/2539407‑2539554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑