Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,557,470	A→G	Y404H (TAC→CAC)	eutB ←	ethanolamine ammonia‑lyase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,557,470	0	A	G	100.0%	120.1 / NA	34	Y404H (TAC→CAC)	eutB	ethanolamine ammonia‑lyase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

CGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGATCAT  >  NC_000913/2557394‑2557542                                                                             |                                                                         cGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTg                                                                      <  1:466345/81‑1 (MQ=255) cGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTg                                                                      <  1:466344/81‑1 (MQ=255)  gTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGtt                                                                       >  2:466402/1‑79 (MQ=255)   ttCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAg                                                                    >  1:466414/1‑81 (MQ=255)     cAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCa                                                                  >  2:466410/1‑81 (MQ=255)      aaaCTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCat                                                                 <  2:466346/81‑1 (MQ=255)         cTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCatgat                                                              <  1:466347/81‑1 (MQ=255)         cTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCatgat                                                              <  1:466348/81‑1 (MQ=255)               tGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGtcatc                                                        <  1:466349/81‑1 (MQ=255)                gACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGtcatca                                                       >  2:466409/1‑81 (MQ=255)                gACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGtcatca                                                       >  2:466408/1‑81 (MQ=255)                   gggCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCAccc                                                    <  1:466350/81‑1 (MQ=255)                   gggCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCAcc                                                     >  2:466401/1‑80 (MQ=255)                    ggCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCa                                                   <  2:466351/81‑1 (MQ=255)                     gcgcAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAg                                                  >  2:466399/1‑81 (MQ=255)                         aGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGc                                              <  1:466352/81‑1 (MQ=255)                           gTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCAt                                            <  2:466353/81‑1 (MQ=255)                                   aCTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCatga                                    <  2:466354/81‑1 (MQ=255)                                    cTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCatgat                                   >  1:466419/1‑81 (MQ=255)                                          cacaGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGtt                             <  1:466355/81‑1 (MQ=255)                                            caGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGc                           <  2:466356/81‑1 (MQ=255)                                            caGTGGCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGc                           <  2:466357/81‑1 (MQ=255)                                                 gCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCt                      <  2:466358/81‑1 (MQ=255)                                                 gCGGTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCt                      <  1:466359/81‑1 (MQ=255)                                                   ggTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGc                    >  2:466428/1‑81 (MQ=255)                                                   ggTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGc                    >  2:466407/1‑81 (MQ=255)                                                   ggTATCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGc                    <  1:466360/81‑1 (MQ=255)                                                      aTCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGt                 >  2:466406/1‑81 (MQ=255)                                                      aTCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGt                 >  1:466411/1‑81 (MQ=255)                                                       tCGTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGt                 <  2:466361/80‑1 (MQ=255)                                                         gTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGc              >  1:466418/1‑81 (MQ=255)                                                         gTGGAATGCGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGc              >  2:466412/1‑81 (MQ=255)                                                                 cGGTGGTCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAgg       <  1:466362/80‑1 (MQ=255)                                                                      gtCTGGTGGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGatcat  >  1:466433/1‑80 (MQ=255)                                                                             |                                                                         CGTTCAAACTCCGGTGACGGGCGCAGGTTGAGTAACTGACGCACAGTGGCGGTATCGTGGAATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGATCAT  >  NC_000913/2557394‑2557542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑