| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,574,860 | A→T | C48* (TGT→TGA) | eutQ ← | putative ethanolamine utilization protein EutQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,574,860 | 0 | A | T | 97.5% | 139.1 / NA | 40 | C48* (TGT→TGA) | eutQ | putative ethanolamine utilization protein EutQ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (21/18); minor base C (1/0); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAC > NC_000913/2574784‑2574934 | cgcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCAt < 1:470118/80‑1 (MQ=255) cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCg < 1:470119/81‑1 (MQ=255) cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCg > 1:470177/1‑81 (MQ=255) tGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGa > 2:470179/1‑81 (MQ=255) cgcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGAt < 2:470120/80‑1 (MQ=255) gcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATgg > 1:470171/1‑81 (MQ=255) gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaaa < 1:470121/81‑1 (MQ=255) gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaa > 2:470192/1‑80 (MQ=255) gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaa < 2:470122/80‑1 (MQ=255) gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTa < 1:470123/79‑1 (MQ=255) tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc > 1:470183/1‑80 (MQ=255) cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAccc < 1:470124/80‑1 (MQ=255) cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc > 2:470169/1‑79 (MQ=255) tGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGa < 1:470125/80‑1 (MQ=255) gTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCATTATATc > 1:470126/1‑81 (MQ=255) ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc < 1:470127/81‑1 (MQ=255) ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc > 1:470187/1‑81 (MQ=255) ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCCCATTCGGTGATGGTAAAACCCAGTAGATCCGc > 1:470195/1‑81 (MQ=255) gacTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCg < 1:470128/78‑1 (MQ=255) gCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACtt < 1:470129/81‑1 (MQ=255) cGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCAc < 2:470130/81‑1 (MQ=255) gCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACg > 2:470176/1‑81 (MQ=255) gCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACg > 2:470186/1‑81 (MQ=255) cTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACgg < 2:470131/81‑1 (MQ=255) cTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACgg > 2:470189/1‑81 (MQ=255) gCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCt < 1:470132/81‑1 (MQ=255) ggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTcc < 2:470133/81‑1 (MQ=255) cGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCgggg > 2:470193/1‑81 (MQ=255) gACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGta < 1:470134/81‑1 (MQ=255) cGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaa > 2:470196/1‑80 (MQ=255) ccGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaataa < 1:470135/81‑1 (MQ=255) gTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATg > 1:470180/1‑81 (MQ=255) gggATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAGTGCTGgcg > 2:470200/1‑81 (MQ=255) gATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470199/1‑81 (MQ=255) aTCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470191/1‑80 (MQ=255) aTCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470182/1‑80 (MQ=255) cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 1:470206/1‑78 (MQ=255) gATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGa < 1:470136/81‑1 (MQ=255) aTTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGaa > 2:470197/1‑81 (MQ=255) aTTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGaa > 1:470190/1‑81 (MQ=255) tcatctCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAc > 2:470185/1‑80 (MQ=255) | CGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAC > NC_000913/2574784‑2574934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |