Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,580,405	A→G	E257G (GAA→GGA)	tktB →	transketolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,580,405	0	A	G	96.5%	97.9 / ‑3.7	29	E257G (GAA→GGA)	tktB	transketolase 2
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (15/13);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGC  >  NC_000913/2580333‑2580479                                                                         |                                                                           tGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgc                                                                    <  2:471217/81‑1 (MQ=255) tGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgc                                                                    <  1:471216/81‑1 (MQ=255) tGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcg                                                                     >  1:471258/1‑80 (MQ=255)   aaaGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgcac                                                                  <  2:471219/81‑1 (MQ=255)   aaaGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgca                                                                   <  1:471218/80‑1 (MQ=255)    aaGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgcacg                                                                 >  1:471271/1‑81 (MQ=255)      gATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgcacggc                                                               >  1:471257/1‑81 (MQ=255)      gATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgcacggc                                                               <  1:471220/81‑1 (MQ=255)         aaGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAggcgcacggcgca                                                            >  1:471268/1‑81 (MQ=255)           gCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGcacc                                                          <  1:471221/81‑1 (MQ=255)             cGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGcaccac                                                        <  1:471223/81‑1 (MQ=255)              gTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACt                                                       >  2:471266/1‑81 (MQ=255)               tCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTg                                                      <  1:471224/81‑1 (MQ=255)               tCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTg                                                      <  2:471225/81‑1 (MQ=255)                     aTTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTggggg                                                  >  2:471270/1‑79 (MQ=255)                     aTTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTggggg                                                  >  1:471259/1‑79 (MQ=255)                        aTCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGgaag                                               >  2:471273/1‑79 (MQ=255)                         tCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGgaag                                               >  1:471261/1‑78 (MQ=255)                           tGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGgaagaag                                            >  1:471265/1‑79 (MQ=255)                                   ggTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCt                                  >  1:471279/1‑81 (MQ=255)                                    gTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTg                                 >  2:471275/1‑81 (MQ=255)                                          ggCTTTGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACg                            <  2:471226/80‑1 (MQ=255)                                               tGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCaaa                       <  1:471227/80‑1 (MQ=255)                                               tGGTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCaa                        <  1:471228/79‑1 (MQ=255)                                                 gTTCGCCGAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAAc                    <  2:471229/81‑1 (MQ=255)                                                        gAATAAAGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTg             >  1:471283/1‑81 (MQ=255)                                                            aaaGCAGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCAc         >  1:471272/1‑81 (MQ=255)                                                                 aGGTAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATcc    >  2:471269/1‑81 (MQ=255)                                                                    tAAGGGAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGc  >  2:471277/1‑80 (MQ=255)                                                                         |                                                                           TGAAAGATAAGCCGTCGCTGATTATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGAAGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGC  >  NC_000913/2580333‑2580479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑