Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,590,292	A→G	M900V (ATG→GTG)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,590,292	0	A	G	100.0%	160.8 / NA	44	M900V (ATG→GTG)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

CTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTC  >  NC_000913/2590214‑2590369                                                                               |                                                                              cTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTg                                                                             >  2:473378/1‑81 (MQ=255) cTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTg                                                                             >  2:473365/1‑81 (MQ=255)   gTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCt                                                                           >  1:473369/1‑81 (MQ=255)    tACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGc                                                                            <  2:473316/79‑1 (MQ=255)      cGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGt                                                                        <  1:473317/81‑1 (MQ=255)      cGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGt                                                                        >  1:473386/1‑81 (MQ=255)      cGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGt                                                                        >  2:473371/1‑81 (MQ=255)       gCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTc                                                                       >  2:473360/1‑81 (MQ=255)         cATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGt                                                                     <  2:473318/81‑1 (MQ=255)               cTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCt                                                               >  1:473370/1‑81 (MQ=255)                   cTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTggg                                                            <  2:473319/80‑1 (MQ=255)                      gtggtATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGt                                                         >  1:473389/1‑80 (MQ=255)                         gtATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAAt                                                      <  1:473320/80‑1 (MQ=255)                             tCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGgcg                                                 >  1:473366/1‑81 (MQ=255)                              ccTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGgcg                                                 <  1:473321/80‑1 (MQ=255)                               cTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGgcgcg                                               <  1:473322/81‑1 (MQ=255)                                  tgtCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCgct                                             <  2:473323/80‑1 (MQ=255)                                      tGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTgg                                        >  1:473383/1‑81 (MQ=255)                                        gCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGctgctg                                         <  1:473325/78‑1 (MQ=255)                                        gCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGc                                       <  2:473324/80‑1 (MQ=255)                                        gCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGc                                       >  1:473382/1‑80 (MQ=255)                                         cTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCaa                                     <  2:473326/81‑1 (MQ=255)                                           gCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAAcc                                   >  1:473390/1‑81 (MQ=255)                                            cGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCt                                  <  1:473327/81‑1 (MQ=255)                                               tGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGa                               >  1:473381/1‑81 (MQ=255)                                                   tGAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATgc                            <  1:473328/80‑1 (MQ=255)                                                    gAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGTTAATCGGCGCGCTGCTGGCAACCTGAATgcgc                          >  2:473377/1‑81 (MQ=255)                                                    gAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATgcgc                          >  1:473387/1‑81 (MQ=255)                                                    gAAAGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATgcgc                          >  2:473398/1‑81 (MQ=255)                                                      aaGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCgg                        >  2:473379/1‑81 (MQ=255)                                                       aGCTGGTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCggg                       >  1:473396/1‑81 (MQ=255)                                                            gTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTg                    <  2:473330/79‑1 (MQ=255)                                                            gTCGGTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGa                  <  2:473329/81‑1 (MQ=255)                                                               ggTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGaaa                <  2:473331/80‑1 (MQ=255)                                                                gTGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAac              >  2:473384/1‑81 (MQ=255)                                                                 tGCCGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAacg             <  1:473332/81‑1 (MQ=255)                                                                    cGTTCTCGGTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAacgacg          >  2:473391/1‑81 (MQ=255)                                                                     gTTCTCGGTAGTGCTGGTCGTGCCGATGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGATGGAAAACGACGt         <  1:473333/81‑1 (MQ=255)                                                                       tctcGGCAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGtt        >  2:473408/1‑80 (MQ=255)                                                                           ggTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACt    <  2:473336/80‑1 (MQ=255)                                                                           ggTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACt    <  1:473334/80‑1 (MQ=255)                                                                           ggTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACt    <  1:473335/80‑1 (MQ=255)                                                                            gTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTc  <  1:473337/81‑1 (MQ=255)                                                                            gTAGTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTc  <  1:473338/81‑1 (MQ=255)                                                                               |                                                                              CTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTC  >  NC_000913/2590214‑2590369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑