Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,592,993	C→T	intergenic (+31/+79)	ypfN → / ← ypfH	UPF0370 protein YpfN/esterase YpfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,592,993	0	C	T	100.0%	132.0 / NA	36	intergenic (+31/+79)	ypfN/ypfH	UPF0370 protein YpfN/esterase YpfH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/11);  total (25/11)

GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGT  >  NC_000913/2592916‑2593068                                                                              |                                                                            gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGgtgt                                                                           >  1:473835/1‑80 (MQ=255)    aCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAc                                                                       <  2:473810/81‑1 (MQ=255)      gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAcgc                                                                     >  2:473864/1‑81 (MQ=255)      gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAAcgc                                                                     >  1:473844/1‑81 (MQ=255)            gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATc                                                               <  2:473811/81‑1 (MQ=255)                acTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCgg                                                            >  1:473867/1‑80 (MQ=255)                  tGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCaa                                                         >  2:473846/1‑81 (MQ=255)                    gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATg                                                       >  2:473847/1‑81 (MQ=255)                       gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGt                                                     >  1:473865/1‑80 (MQ=255)                       gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGt                                                     >  1:473862/1‑80 (MQ=255)                              gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATg                                             >  2:473849/1‑81 (MQ=255)                                tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                           >  1:473871/1‑81 (MQ=255)                                  aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                          <  1:473812/80‑1 (MQ=255)                                      gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGc                                     >  2:473850/1‑81 (MQ=255)                                      gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGc                                     >  2:473848/1‑81 (MQ=255)                                       aagaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCg                                    <  2:473813/81‑1 (MQ=255)                                         gaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAc                                  <  1:473814/81‑1 (MQ=255)                                          aagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACg                                 >  1:473852/1‑81 (MQ=255)                                           agTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGc                                >  1:473854/1‑81 (MQ=255)                                             tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt                               >  2:473857/1‑80 (MQ=255)                                               aGCTGAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAc                            <  2:473815/81‑1 (MQ=255)                                                   gAGTGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt                        >  1:473866/1‑81 (MQ=255)                                                      tGCTCAACCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCt                      >  1:473869/1‑80 (MQ=255)                                                           aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCa                 <  2:473816/80‑1 (MQ=255)                                                           aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAg                >  1:473876/1‑81 (MQ=255)                                                           aaCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAg                >  1:473856/1‑81 (MQ=255)                                                            aCCGTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg               >  1:473855/1‑81 (MQ=255)                                                               gTAGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGc              >  1:473886/1‑79 (MQ=255)                                                                 aGGCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAgg          >  2:473858/1‑81 (MQ=255)                                                                  ggCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt         <  2:473817/81‑1 (MQ=255)                                                                   gCCGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGt         >  1:473853/1‑80 (MQ=255)                                                                     cGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt      >  1:473863/1‑81 (MQ=255)                                                                     cGGATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt      >  1:473868/1‑81 (MQ=255)                                                                      ggATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc     <  1:473818/81‑1 (MQ=255)                                                                       gATAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCt    >  2:473861/1‑81 (MQ=255)                                                                        aTAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg   <  1:473819/81‑1 (MQ=255)                                                                         tAAGGTGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGt  <  2:473820/81‑1 (MQ=255)                                                                              |                                                                            GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGT  >  NC_000913/2592916‑2593068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑