Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,485	A→G	I56I (ATT→ATC)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,485	0	A	G	100.0%	90.2 / NA	26	I56I (ATT→ATC)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

TTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATC  >  NC_000913/2638409‑2638560                                                                             |                                                                            ttACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGAt                                                                           <  2:483060/79‑1 (MQ=255) ttACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGAt                                                                           <  2:483059/79‑1 (MQ=255)  tACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGcc                                                                        >  1:483100/1‑81 (MQ=255)    cTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCg                                                                       <  1:483062/80‑1 (MQ=255)    cTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGc                                                                      <  1:483061/81‑1 (MQ=255)       aaCCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTAc                                                                   >  1:483104/1‑81 (MQ=255)         ccAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCa                                                                 <  1:483063/81‑1 (MQ=255)                 gTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGt                                                         <  1:483064/81‑1 (MQ=255)                    cGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCt                                                      <  1:483065/81‑1 (MQ=255)                         cATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCAc                                                  <  2:483066/80‑1 (MQ=255)                         cATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGCTGCCGATGCCGCTACCAACGGAAGTGCTCCAc                                                  <  2:795937/80‑1 (MQ=255)                          aTAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGc                                                >  2:483094/1‑81 (MQ=255)                           tAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGcc                                               <  2:483067/81‑1 (MQ=255)                             gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGt                                             <  2:483068/81‑1 (MQ=255)                             gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGt                                             <  2:483069/81‑1 (MQ=255)                              aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTg                                            >  1:483108/1‑81 (MQ=255)                              aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTg                                            <  2:483070/81‑1 (MQ=255)                                 aCGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTc                                         >  2:483116/1‑81 (MQ=255)                                      gTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCg                                     >  1:483110/1‑80 (MQ=255)                                                gCCGGATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGttt                          >  2:483111/1‑81 (MQ=255)                                                    gATGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCaa                      >  2:483107/1‑81 (MQ=255)                                                      tGAAGATTGGAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACg                    >  1:483102/1‑81 (MQ=255)                                                              ggAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCaa            >  2:483113/1‑81 (MQ=255)                                                               gAATAGAAGTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCaa            <  1:483071/80‑1 (MQ=255)                                                                       gTTGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACAt   >  2:483112/1‑81 (MQ=255)                                                                        ttGCCGATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATc  <  2:483072/81‑1 (MQ=255)                                                                             |                                                                            TTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATC  >  NC_000913/2638409‑2638560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑