Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,647,072	T→C	D85G (GAT→GGT)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,647,072	0	T	C	100.0%	116.3 / NA	33	D85G (GAT→GGT)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

CCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGT  >  NC_000913/2646998‑2647147                                                                           |                                                                            ccGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCa                                                                        >  2:484925/1‑80 (MQ=255) ccGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCa                                                                        <  2:484887/80‑1 (MQ=255)  cGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATa                                                                      <  1:484888/81‑1 (MQ=255)   gAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATaa                                                                     <  1:484889/81‑1 (MQ=255)        gagaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGAt                                                                >  1:484922/1‑81 (MQ=255)                gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAgcg                                                           <  2:484890/78‑1 (MQ=255)                gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAg                                                             <  1:484891/76‑1 (MQ=255)                   aTGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTc                                                       >  1:484937/1‑79 (MQ=255)                      ctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGt                                                  <  1:484892/81‑1 (MQ=255)                         cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc                                               <  1:484893/81‑1 (MQ=255)                         cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc                                               <  1:484895/81‑1 (MQ=255)                         cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAAc                                               <  2:484894/81‑1 (MQ=255)                             cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTgg                                           <  2:484896/81‑1 (MQ=255)                             cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTg                                            >  2:484934/1‑80 (MQ=255)                                    gagaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACa                                    >  1:484924/1‑81 (MQ=255)                                     agaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACAt                                   >  1:484943/1‑81 (MQ=255)                                         tGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc                               <  1:484897/81‑1 (MQ=255)                                               cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTg                          <  1:484900/80‑1 (MQ=255)                                               cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTg                          <  2:484899/80‑1 (MQ=255)                                               cACCCCCGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGt                         >  2:484929/1‑81 (MQ=255)                                                ccccccGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGt                         >  2:484928/2‑80 (MQ=255)                                                  ccccGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTAc                      >  2:800540/1‑81 (MQ=255)                                                     cGGATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCgg                   <  1:484901/81‑1 (MQ=255)                                                      ggATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa                  >  1:484944/1‑81 (MQ=255)                                                       gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa                  >  2:484926/1‑80 (MQ=255)                                                       gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa                  >  2:484933/1‑80 (MQ=255)                                                       gATGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGa                  >  1:484949/1‑80 (MQ=255)                                                        aTGCTTCCAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGAt                 >  1:484927/1‑80 (MQ=255)                                                              ccAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGcc           <  1:484902/80‑1 (MQ=255)                                                               cAGAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGc            >  2:484942/1‑78 (MQ=255)                                                                 gAACCAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGAt       >  1:484938/1‑81 (MQ=255)                                                                    ccAGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGcc    <  2:484903/81‑1 (MQ=255)                                                                      aGCGACCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGt  <  1:484904/81‑1 (MQ=255)                                                                           |                                                                            CCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGT  >  NC_000913/2646998‑2647147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑