Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	306,370	C→T	G150S (GGC→AGC)	ecpD ←	fimbrial adhesin EcpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	306,370	0	C	T	100.0%	77.1 / NA	22	G150S (GGC→AGC)	ecpD	fimbrial adhesin EcpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

TGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCAT  >  NC_000913/306295‑306439                                                                            |                                                                      tgctgcCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTgg                                                                  >  1:66804/1‑81 (MQ=255)   ctgcCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGTCGCTGCTgg                                                                  <  1:66766/79‑1 (MQ=255)      ccGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCAcc                                                             <  2:66767/81‑1 (MQ=255)        gACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGt                                                           <  2:66768/81‑1 (MQ=255)            ggCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTcgcg                                                       >  2:66797/1‑81 (MQ=255)                tttGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCAt                                                   <  1:66769/81‑1 (MQ=255)                   gttgCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATaa                                                 <  2:66770/80‑1 (MQ=255)                       cATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATc                                             <  1:66771/80‑1 (MQ=255)                                      cgcgTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCggtggt                             <  2:66772/81‑1 (MQ=255)                                       gcgTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGtt                            <  1:66773/81‑1 (MQ=255)                                       gcgTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGtt                            >  2:66809/1‑81 (MQ=255)                                        cgTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGttt                           <  1:66774/81‑1 (MQ=255)                                        cgTCTGACAACATGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGttt                           >  2:66815/1‑81 (MQ=255)                                                aacaTGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAg                    >  1:66807/1‑80 (MQ=255)                                                  caTGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGa                  >  2:66808/1‑80 (MQ=255)                                                   aTGCCGTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGAt                 >  1:66806/1‑80 (MQ=255)                                                        gTGCATCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACt           >  1:66803/1‑81 (MQ=255)                                                            aTCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACtggtg       >  2:66805/1‑81 (MQ=255)                                                             tCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACtggtgg      <  2:66775/81‑1 (MQ=255)                                                             tCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACtggtgg      <  2:66776/81‑1 (MQ=255)                                                             tCCACTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACtggtgg      <  2:66777/81‑1 (MQ=255)                                                                 cTTCGCGCCGCTGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCat  <  1:66778/81‑1 (MQ=255)                                                                            |                                                                      TGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCAT  >  NC_000913/306295‑306439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑