Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,676	A→T	F71I (TTT→ATT)	iscA ←	iron‑sulfur cluster insertion protein IscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,676	0	A	T	100.0%	132.3 / NA	37	F71I (TTT→ATT)	iscA	iron‑sulfur cluster insertion protein IscA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/20);  total (17/20)

GTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTC  >  NC_000913/2659605‑2659751                                                                        |                                                                            gTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGct                                                                    <  2:487457/81‑1 (MQ=255) gTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGct                                                                    >  1:487508/1‑81 (MQ=255)      ggTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGc                                                               >  2:487527/1‑81 (MQ=255)               aTTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCa                                                      <  1:487458/81‑1 (MQ=255)                tttAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCAc                                                     <  1:487459/81‑1 (MQ=255)                   aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGAc                                                  >  1:487510/1‑81 (MQ=255)                    aaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGAc                                                  <  2:487460/80‑1 (MQ=255)                    aaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACt                                                 >  2:487509/1‑81 (MQ=255)                     aCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACtt                                                <  2:487461/81‑1 (MQ=255)                            gTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGcc                                         >  2:487506/1‑81 (MQ=255)                              tCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt                                       >  1:487504/1‑81 (MQ=255)                               cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCttt                                      >  1:487516/1‑81 (MQ=255)                               cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt                                       <  2:487463/80‑1 (MQ=255)                               cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt                                       <  2:487462/80‑1 (MQ=255)                                aGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCttt                                      <  2:487464/80‑1 (MQ=255)                                   ccTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCt                                  >  1:487523/1‑81 (MQ=255)                                    cttcttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCtt                                 >  2:487507/1‑81 (MQ=255)                                    cttcttTTACGAAGTCCAGCTGCGTACCGTCCAGAATCTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCtt                                 <  1:487465/81‑1 (MQ=255)                                       cttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCa                               <  2:487466/80‑1 (MQ=255)                                       cttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCa                               >  2:487514/1‑80 (MQ=255)                                        ttttACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCaaa                             >  1:487512/1‑81 (MQ=255)                                        ttttACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCaaa                             >  1:487511/1‑81 (MQ=255)                                           tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAacac                          >  1:487546/1‑81 (MQ=255)                                           tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca                           <  1:487468/80‑1 (MQ=255)                                           tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca                           <  1:487467/80‑1 (MQ=255)                                           tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca                           <  2:487469/80‑1 (MQ=255)                                            aCGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAacac                          <  2:487470/80‑1 (MQ=255)                                              gAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGAt                       >  2:487519/1‑81 (MQ=255)                                                 gTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTc                    <  2:487471/81‑1 (MQ=255)                                                    cAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCtt                  <  2:487472/80‑1 (MQ=255)                                                     aGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc                <  1:487473/81‑1 (MQ=255)                                                     aGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc                <  1:487474/81‑1 (MQ=255)                                                       cTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCgg              <  1:487475/81‑1 (MQ=255)                                                           gTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTc          <  1:487476/81‑1 (MQ=255)                                                           gTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTc          >  1:487513/1‑81 (MQ=255)                                                                gTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTc     >  2:487526/1‑81 (MQ=255)                                                                    aGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTc  >  2:487520/1‑80 (MQ=255)                                                                        |                                                                            GTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTC  >  NC_000913/2659605‑2659751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑