Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,677,674	C→A	D230Y (GAT→TAT)	yphE ←	putative ABC transporter ATP‑binding protein YphE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,677,674	0	C	A	100.0%	102.2 / NA	29	D230Y (GAT→TAT)	yphE	putative ABC transporter ATP‑binding protein YphE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/13);  total (16/13)

CGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC  >  NC_000913/2677597‑2677745                                                                              |                                                                        cGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcc                                                                      >  2:491353/1‑81 (MQ=255)  ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc                                                                     >  2:491365/1‑81 (MQ=255)  ggCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagccc                                                                     <  2:491333/81‑1 (MQ=255)   gcgcAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATagcccg                                                                    >  2:491381/1‑81 (MQ=255)         tatCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCt                                                              <  1:491335/81‑1 (MQ=255)           tCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTg                                                             <  1:491337/80‑1 (MQ=255)           tCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGa                                                            <  1:491336/81‑1 (MQ=255)                gTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATc                                                       >  1:491379/1‑81 (MQ=255)                gTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATc                                                       <  2:491338/81‑1 (MQ=255)                    tCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCgcgc                                                   <  1:491339/81‑1 (MQ=255)                      gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCa                                                  >  1:491399/1‑80 (MQ=255)                      gcgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt                                                 <  1:491340/81‑1 (MQ=255)                       cgcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCAt                                                 >  1:491394/1‑80 (MQ=255)                        gcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATaa                                               >  2:491389/1‑81 (MQ=255)                        gcCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATaa                                               <  2:491341/81‑1 (MQ=255)                          ccGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACg                                             >  1:491392/1‑81 (MQ=255)                           cGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACgg                                            >  2:491393/1‑81 (MQ=255)                              gCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTgg                                         >  1:491388/1‑81 (MQ=255)                               cATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGc                                        <  1:491342/81‑1 (MQ=255)                                aTCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGca                                       >  1:491382/1‑81 (MQ=255)                                        cacaATATGATGCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACaggagg                                >  2:491376/1‑80 (MQ=255)                                              atgatgCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATgcg                         >  2:491390/1‑81 (MQ=255)                                                   gCGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGaa                    >  2:491374/1‑81 (MQ=255)                                                    cGTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAAt                   <  1:491343/81‑1 (MQ=255)                                                     gTGGAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAAtt                  >  2:491386/1‑81 (MQ=255)                                                        gAGGTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttct               <  1:491344/81‑1 (MQ=255)                                                          ggTGTTTTCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATttcttc             <  2:491345/81‑1 (MQ=255)                                                              ttttCGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATc         >  2:491385/1‑81 (MQ=255)                                                                  cGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCgg      <  1:491346/80‑1 (MQ=255)                                                                  cGAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGt     >  1:491403/1‑81 (MQ=255)                                                                   gAGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTg    >  1:491406/1‑81 (MQ=255)                                                                    aGCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTgg   >  1:491373/1‑81 (MQ=255)                                                                     gCATCACATAGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGc  <  2:491347/81‑1 (MQ=255)                                                                              |                                                                        CGGCGCAATATCAACGTGATCGCGCCCGAGCATCAGCGACACAATATGATGCGTGGAGGTGTTTTCGAGCATCACATCGCCCGCCACCTGACCATCGCGCATAACGGTGGCACAGGAGGCAATGCGGCGAATTTCTTCCATCCGGTGGC  >  NC_000913/2677597‑2677745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑