Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,712,311	G→A	L152L (CTG→TTG)	yfiC ←	tRNA m(6)A37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,712,311	0	G	A	100.0%	121.8 / NA	33	L152L (CTG→TTG)	yfiC	tRNA m(6)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/9);  total (24/9)

AAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGAT  >  NC_000913/2712234‑2712388                                                                              |                                                                              aaaaCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTcaacaa                                                                            >  1:498386/1‑81 (MQ=255)  aaaCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCaacaac                                                                           <  1:498363/81‑1 (MQ=255)  aaaCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCaacaac                                                                           >  2:498389/1‑81 (MQ=255)    aCCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGa                                                                         >  1:498388/1‑81 (MQ=255)     ccATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGAt                                                                        <  1:498364/81‑1 (MQ=255)     ccATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGAt                                                                        <  1:498365/81‑1 (MQ=255)         tACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGt                                                                    >  2:498414/1‑81 (MQ=255)         tACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGt                                                                    >  2:498413/1‑81 (MQ=255)          aCCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGt                                                                    >  2:498418/1‑80 (MQ=255)            cAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGAt                                                                 >  1:498402/1‑81 (MQ=255)             aaTCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGAt                                                                 >  1:498398/1‑80 (MQ=255)                  gCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCg                                                           >  1:498397/1‑81 (MQ=255)                      tGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGt                                                       >  2:498404/1‑81 (MQ=255)                           gcaCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGGTGGGTGATCAAGCGTAGTGGtat                                                  <  2:498366/81‑1 (MQ=255)                             aCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAg                                                >  2:498423/1‑81 (MQ=255)                             aCTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAg                                                >  1:498403/1‑81 (MQ=255)                                aCGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGc                                               >  2:498412/1‑79 (MQ=255)                                aCGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGc                                               >  2:498408/1‑79 (MQ=255)                                         aaTCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTcc                                    >  1:498420/1‑81 (MQ=255)                                          aTCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGT‑GTATAGCGAGCCTGTTCCCg                                  >  2:498417/1‑81 (MQ=255)                                             ccTCTTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGtt                                >  1:498415/1‑81 (MQ=255)                                               tctTCGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGtt                                >  1:498425/1‑79 (MQ=255)                                                ctTCGGTAATGCACTCCGCCGCACAGGTCAACAGCGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAg                             >  1:498424/1‑81 (MQ=255)                                                   cGGTAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGgtg                          >  2:498421/1‑81 (MQ=255)                                                      tAATGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCg                       <  2:498367/81‑1 (MQ=255)                                                       aaTGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTc                        >  1:498407/1‑79 (MQ=255)                                                       aaTGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTc                        >  2:498368/1‑79 (MQ=255)                                                        aTGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGgtgtagca                     >  1:498429/1‑77 (MQ=255)                                                         tGCACTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGc                    <  1:498368/81‑1 (MQ=255)                                                            aCTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCAct                 >  1:498410/1‑81 (MQ=255)                                                            aCTCCGCCGCACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCAct                 <  1:498369/81‑1 (MQ=255)                                                                 gccgcACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACt            >  1:498422/1‑81 (MQ=255)                                                                  ccgcACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTc           >  2:498409/1‑81 (MQ=255)                                                                  ccgcACAGGTCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTc           <  1:498370/81‑1 (MQ=255)                                                                           tCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGAt  <  2:498372/81‑1 (MQ=255)                                                                           tCAACAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGAt  <  1:498371/81‑1 (MQ=255)                                                                              |                                                                              AAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATCAAGCGTAGTGGTATAGCGAGCCTGTTCCCGTTGAGGTGTCGAGCACTCTACTCCCTGCTGAT  >  NC_000913/2712234‑2712388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑