Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,750,090	C→T	intergenic (+30/+25)	yfjD → / ← grpE	putative inner membrane protein/nucleotide exchange factor GrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,750,090	0	C	T	100.0%	124.1 / NA	34	intergenic (+30/+25)	yfjD/grpE	putative inner membrane protein/nucleotide exchange factor GrpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

GGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC  >  NC_000913/2750012‑2750157                                                                               |                                                                    ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCg                                                                   >  1:506226/1‑81 (MQ=255)   tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTg                                                                 <  2:506193/81‑1 (MQ=255)       aGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAat                                                              >  1:506243/1‑80 (MQ=255)        gTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAatta                                                            >  1:506228/1‑81 (MQ=255)          ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAattat                                                           >  1:506221/1‑80 (MQ=255)          ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAattat                                                           >  1:506229/1‑80 (MQ=255)           tttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAattatt                                                          <  1:506194/80‑1 (MQ=255)              ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACg                                                       <  1:506195/80‑1 (MQ=255)              ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACg                                                       >  1:506241/1‑80 (MQ=255)               cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGaa                                                     <  1:506196/81‑1 (MQ=255)                tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGa                                                      >  2:506235/1‑79 (MQ=255)                   gAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAg                                                   >  2:506233/1‑79 (MQ=255)                        cGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAAt                                            <  2:506197/81‑1 (MQ=255)                          cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTa                                          <  2:506198/81‑1 (MQ=255)                            gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAg                                        >  1:506227/1‑81 (MQ=255)                             cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTaa                                         <  2:506199/79‑1 (MQ=255)                              gcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCt                                      >  2:506230/1‑81 (MQ=255)                               cgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                     <  2:506200/81‑1 (MQ=255)                                  gagTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                  >  1:506244/1‑81 (MQ=255)                                    gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                >  1:506252/1‑81 (MQ=255)                                    gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                <  2:506201/81‑1 (MQ=255)                                          cGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                           >  2:506225/1‑80 (MQ=255)                                           ggAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                         <  2:506202/81‑1 (MQ=255)                                            gAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAc                        <  2:506203/81‑1 (MQ=255)                                            gAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAc                        <  2:506204/81‑1 (MQ=255)                                              gTAACGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                      <  2:506205/81‑1 (MQ=255)                                                aaCGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGt                     >  2:506231/1‑80 (MQ=255)                                                 aCGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa                   >  1:506245/1‑81 (MQ=255)                                                  cGAAAACGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                  <  2:506206/81‑1 (MQ=255)                                                     aaaCGGCCCGGCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt               >  1:506232/1‑81 (MQ=255)                                                              ggCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCAc       >  1:506239/1‑80 (MQ=255)                                                              ggCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg      <  2:506207/81‑1 (MQ=255)                                                               gCATTCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg      >  2:506236/1‑80 (MQ=255)                                                                  ttCGCATGCAGGGTCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc  <  1:506208/81‑1 (MQ=255)                                                                               |                                                                    GGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC  >  NC_000913/2750012‑2750157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑