Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,434	T→C	S202P (TCT→CCT)	nadK →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,434	0	T	C	100.0%	86.4 / NA	25	S202P (TCT→CCT)	nadK	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

CTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAG  >  NC_000913/2751357‑2751507                                                                              |                                                                          cTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCccctc                                                                        >  2:506516/1‑81 (MQ=255)   gATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTg                                                                      >  1:506520/1‑81 (MQ=255)         cTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGc                                                                 >  1:506528/1‑80 (MQ=255)            attattTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGAt                                                              >  2:506532/1‑80 (MQ=255)             ttattTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTa                                                            <  2:506473/81‑1 (MQ=255)               attTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTAcc                                                          >  1:506524/1‑81 (MQ=255)                tttCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTAccc                                                         <  1:506474/81‑1 (MQ=255)                    gACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGt                                                     <  1:506475/81‑1 (MQ=255)                      cGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGc                                                   >  2:506527/1‑81 (MQ=255)                       gCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGcc                                                  >  2:506525/1‑81 (MQ=255)                       gCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGcc                                                  <  2:506476/81‑1 (MQ=255)                         cAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCa                                                >  1:506523/1‑81 (MQ=255)                             aGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGtt                                            >  2:506538/1‑81 (MQ=255)                             aGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGtt                                            >  2:506517/1‑81 (MQ=255)                                cTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTccc                                         <  2:506477/81‑1 (MQ=255)                                    aCCGCCTATTCCCTCTCTGCAGGCGGTCCTAATCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCAt                                     <  2:506478/81‑1 (MQ=255)                                            ttCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTc                             <  1:506479/81‑1 (MQ=255)                                              cccTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCa                            <  2:506480/80‑1 (MQ=255)                                               ccTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAg                           >  2:506535/1‑80 (MQ=255)                                               ccTCTCTGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGc                          <  2:506481/81‑1 (MQ=255)                                                   tctGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCAc                        <  1:506482/79‑1 (MQ=255)                                                     tGCAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGAc                     >  2:506533/1‑80 (MQ=255)                                                       cAGGCGGTCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGAc                     <  1:506483/78‑1 (MQ=255)                                                              tCCTATTCTGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCAt           <  1:506484/81‑1 (MQ=255)                                                                      tGACCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcag     >  1:506536/1‑79 (MQ=255)                                                                        aCCCCCCCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAAcagcag  <  2:506485/80‑1 (MQ=255)                                                                              |                                                                          CTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGCCTATTCCCTCTCTGCAGGCGGTCCTATTCTGACCCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAG  >  NC_000913/2751357‑2751507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑