Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,779,727	A→G	N1000N (AAT→AAC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,779,727	0	A	G	100.0%	116.6 / NA	33	N1000N (AAT→AAC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/21);  total (12/21)

TATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTACCTGCGT  >  NC_000913/2779651‑2779800                                                                             |                                                                          tATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTAt                                                                       >  1:511408/1‑81 (MQ=255)  aTCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATc                                                                      >  2:511423/1‑81 (MQ=255)   tCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATcc                                                                     <  1:511362/81‑1 (MQ=255)    cGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATccc                                                                    >  2:511419/1‑81 (MQ=255)     gACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCg                                                                   >  1:511413/1‑81 (MQ=255)         ccATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGc                                                               <  2:511364/81‑1 (MQ=255)         ccATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGc                                                               <  2:511363/81‑1 (MQ=255)          cATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCg                                                              <  1:511365/81‑1 (MQ=255)          cATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCg                                                              >  2:511412/1‑81 (MQ=255)               ccaagagTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCAt                                                          <  2:511366/77‑1 (MQ=255)                 cAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTaa                                                       <  1:511367/81‑1 (MQ=255)                  agagTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAAc                                                      <  2:511368/81‑1 (MQ=255)                              gcgcTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTcc                                           <  2:511369/80‑1 (MQ=255)                              gcgcTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTcc                                           <  2:511370/80‑1 (MQ=255)                                gcTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGt                                        >  1:511426/1‑81 (MQ=255)                                gcTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGt                                        <  1:511371/81‑1 (MQ=255)                                 cTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTc                                       <  1:511372/81‑1 (MQ=255)                                       gTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCTCCa                                 >  2:511433/1‑81 (MQ=255)                                             cGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGAcc                           <  1:511373/81‑1 (MQ=255)                                             cGGACCGCCCCTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCACTAACGACAGGCTTCCTGTCGCGCCACTGAcc                           >  2:511441/1‑81 (MQ=255)                                                    ccccTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCAt                    <  2:511374/81‑1 (MQ=255)                                                     cccTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATc                   <  1:511375/81‑1 (MQ=255)                                                     cccTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATc                   >  1:511425/1‑81 (MQ=255)                                                      ccTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATcc                  <  2:511376/81‑1 (MQ=255)                                                      ccTTCGAGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATcc                  <  2:511377/81‑1 (MQ=255)                                                            aGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGc            <  1:511378/81‑1 (MQ=255)                                                            aGTTTAACTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGc            >  1:511420/1‑81 (MQ=255)                                                                 aaCTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTAcc       >  1:511445/1‑81 (MQ=255)                                                                 aaCTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTAc        <  2:511379/80‑1 (MQ=255)                                                                  aCTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTAcc       <  2:511380/80‑1 (MQ=255)                                                                   cTGGCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTACCTg     <  2:511381/81‑1 (MQ=255)                                                                     ggCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTACCTGCg   >  1:511427/1‑81 (MQ=255)                                                                      gCGTAACGTTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTACCTGCGt  <  2:511382/81‑1 (MQ=255)                                                                             |                                                                          TATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCGTCATTAACGACAGGCTTCCTGTCGCGCCACTGACCGATGCATCCGCCAGCGTACCTGCGT  >  NC_000913/2779651‑2779800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑