Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,613	T→A	I107I (ATT→ATA)	rihC →	ribonucleoside hydrolase RihC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,613	0	T	A	100.0%	169.3 / NA	46	I107I (ATT→ATA)	rihC	ribonucleoside hydrolase RihC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/21);  total (25/21)

CGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACT  >  NC_000913/27535‑27690                                                                               |                                                                              cGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACg                                                                             <  2:6003/81‑1 (MQ=255)  ggCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACgg                                                                            >  1:6047/1‑81 (MQ=255)   gCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACggg                                                                           <  1:6004/81‑1 (MQ=255)    cGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGa                                                                          >  1:6075/1‑81 (MQ=255)     gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                         <  2:6005/81‑1 (MQ=255)     gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                         >  1:6074/1‑81 (MQ=255)     gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                         >  1:6067/1‑81 (MQ=255)     gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGAt                                                                         >  1:6069/1‑81 (MQ=255)       aTCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGc                                                                       >  1:6063/1‑81 (MQ=255)         cGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGccc                                                                     >  2:6076/1‑81 (MQ=255)            gAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGa                                                                  <  2:6006/81‑1 (MQ=255)            gAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGa                                                                  >  1:6079/1‑81 (MQ=255)             aaTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGAt                                                                 <  1:6007/81‑1 (MQ=255)             aaTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGAt                                                                 >  1:6051/1‑81 (MQ=255)               tggctggcTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGc                                                               <  2:6009/81‑1 (MQ=255)               tggctggcTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGc                                                               <  2:6008/81‑1 (MQ=255)                ggctggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCg                                                              >  2:6065/1‑81 (MQ=255)                ggctggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCg                                                              >  2:894249/1‑81 (MQ=255)                 gctggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGt                                                             >  1:6053/1‑81 (MQ=255)                 gctggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGt                                                             <  2:6010/81‑1 (MQ=255)                     gctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGc                                                           <  2:6011/79‑1 (MQ=255)                     gctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCAc                                                         >  2:6061/1‑81 (MQ=255)                     gctACGACTTTGTAGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGc                                                           <  1:6012/79‑1 (MQ=255)                        aCGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCAc                                                         >  2:6058/1‑78 (MQ=255)                          gACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCagag                                                    >  1:6081/1‑81 (MQ=255)                                 ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                              <  1:6013/80‑1 (MQ=255)                                  tgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTAc                                            >  2:6086/1‑81 (MQ=255)                                    aGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTAcc                                           <  1:537058/80‑1 (MQ=255)                                     gCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCt                                         <  2:6014/81‑1 (MQ=255)                                       acaACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtgg                                       >  1:6059/1‑81 (MQ=255)                                           ccGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGc                                   >  1:6077/1‑81 (MQ=255)                                            cGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGc                                   >  1:6094/1‑80 (MQ=255)                                                    gCTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGccc                          <  2:6015/81‑1 (MQ=255)                                                     cTCGGGATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCg                         >  1:6064/1‑81 (MQ=255)                                                        gggATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGtt                       <  2:6017/80‑1 (MQ=255)                                                        gggATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGtt                       >  2:6072/1‑80 (MQ=255)                                                        gggATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTa                      <  1:6018/81‑1 (MQ=255)                                                        gggATACCGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTa                      <  2:6016/81‑1 (MQ=255)                                                              ccGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAat                <  1:6019/81‑1 (MQ=255)                                                               cGGCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAata               <  1:6020/81‑1 (MQ=255)                                                                ggCGTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAatat              >  1:6066/1‑81 (MQ=255)                                                                   gTTTCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTgc           >  1:6071/1‑81 (MQ=255)                                                                      tCTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt        >  2:6090/1‑81 (MQ=255)                                                                       cTGGCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt        <  1:6021/80‑1 (MQ=255)                                                                          gCGATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGtt     <  2:6022/80‑1 (MQ=255)                                                                            gATACGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCTTCGGCCCGTTAACCAATATTGCGCTGTTACt  >  2:6073/1‑81 (MQ=255)                                                                               |                                                                              CGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACT  >  NC_000913/27535‑27690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑