Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,782,394	G→A	D111D (GAC→GAT)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,782,394	0	G	A	100.0%	102.2 / NA	29	D111D (GAC→GAT)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

TTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATC  >  NC_000913/2782321‑2782471                                                                          |                                                                              ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAt                                                                       >  2:511950/1‑81 (MQ=255)  tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAtt                                                                      >  2:535785/1‑81 (MQ=255)   ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAAtt                                                                      <  2:511918/80‑1 (MQ=255)       tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTg                                                                 >  2:511947/1‑81 (MQ=255)        gCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGt                                                                >  1:511954/1‑81 (MQ=255)          tGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCt                                                              >  2:511955/1‑81 (MQ=255)            aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCt                                                              <  1:511919/79‑1 (MQ=255)              aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCa                                                          >  1:511920/1‑81 (MQ=255)                ccccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCAcc                                                        <  2:511921/81‑1 (MQ=255)                   cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGtt                                                     <  1:511922/81‑1 (MQ=255)                       ataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAAt                                                  <  1:511923/80‑1 (MQ=255)                       ataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATg                                                 >  2:511968/1‑81 (MQ=255)                        taAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATgg                                                >  1:511951/1‑81 (MQ=255)                         aaaCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGt                                               >  2:511958/1‑81 (MQ=255)                             gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGta                                           <  1:511924/81‑1 (MQ=255)                                     tGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                   >  2:511961/1‑81 (MQ=255)                                     tGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                   >  1:511962/1‑81 (MQ=255)                                      gCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                   <  1:511925/80‑1 (MQ=255)                                          ctctCGATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcc                              <  2:511926/81‑1 (MQ=255)                                               gATTGTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCcaca                         <  1:511927/81‑1 (MQ=255)                                                   gTCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaaa                      <  2:511928/80‑1 (MQ=255)                                                    tCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAAtt                    <  1:511930/81‑1 (MQ=255)                                                    tCGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAAt                     <  2:511929/80‑1 (MQ=255)                                                     cGTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTg                   >  1:511957/1‑81 (MQ=255)                                                      gTACCTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGt                  >  1:511953/1‑81 (MQ=255)                                                          cTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt               >  1:511960/1‑80 (MQ=255)                                                          cTGTGGAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt               >  1:511959/1‑80 (MQ=255)                                                               gAAATA‑CCCCATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc         <  2:511931/81‑1 (MQ=255)                                                                       cccATCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATc  >  1:511956/1‑81 (MQ=255)                                                                          |                                                                              TTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATC  >  NC_000913/2782321‑2782471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑