Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,666	T→A	V102E (GTG→GAG)	srlB →	sorbitol‑specific PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,666	0	T	A	100.0%	135.6 / NA	37	V102E (GTG→GAG)	srlB	sorbitol‑specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/16);  total (21/16)

AACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATC  >  NC_000913/2827590‑2827743                                                                             |                                                                              aaCCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCa                                                                           <  2:520653/81‑1 (MQ=255)   ccTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAgg                                                                         >  1:520710/1‑81 (MQ=255)    cTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGaggcaggc                                                                        >  2:520699/1‑81 (MQ=255)      tCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGccc                                                                      >  2:520705/1‑81 (MQ=255)        gcgAACTGGGTCATATCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCt                                                                     <  1:520654/80‑1 (MQ=255)         cgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGt                                                                   >  2:520714/1‑81 (MQ=255)           aaCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTcc                                                                 >  1:520703/1‑81 (MQ=255)                    aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGata                                                        >  1:520719/1‑81 (MQ=255)                    aTGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGat                                                         <  1:520655/80‑1 (MQ=255)                      gTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATc                                                      >  2:520708/1‑81 (MQ=255)                         aCCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATcgcg                                                   <  2:520656/81‑1 (MQ=255)                          cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCgcgc                                                  <  2:520657/81‑1 (MQ=255)                          cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCgcgc                                                  >  2:520701/1‑81 (MQ=255)                              gcgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCgg                                               >  2:520720/1‑80 (MQ=255)                                gcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGAt                                            >  1:520704/1‑81 (MQ=255)                                 cTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc                                           <  1:520658/81‑1 (MQ=255)                                 cTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATc                                           <  2:520659/81‑1 (MQ=255)                                     gATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtt                                       >  2:520706/1‑81 (MQ=255)                                       tGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGtttt                                     >  2:520721/1‑81 (MQ=255)                                         gTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGa                                   <  1:520660/81‑1 (MQ=255)                                             aaaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGtt                               >  1:520717/1‑81 (MQ=255)                                              aaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGttt                              >  1:520707/1‑81 (MQ=255)                                              aaCGAAGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGtt                               <  1:520661/80‑1 (MQ=255)                                                   aGCGGAATTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATc                         >  1:520724/1‑81 (MQ=255)                                                        aaTTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTa                    <  2:520662/81‑1 (MQ=255)                                                         aTTTCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGtt                     >  1:520713/1‑79 (MQ=255)                                                            tCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAgg                 <  2:520663/80‑1 (MQ=255)                                                            tCCGGGCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAg                  >  2:520725/1‑79 (MQ=255)                                                               gggCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGTAGTTTGAATCTGTTAAGGAGt              >  1:520715/1‑80 (MQ=255)                                                               gggCACTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGCTTTGAAGTTTGAATCTGTTAAGGAGt              >  1:520709/1‑80 (MQ=255)                                                                    cTGTCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTaaaaaa        <  1:520664/81‑1 (MQ=255)                                                                       tCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAAt       <  2:520666/79‑1 (MQ=255)                                                                       tCCATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATg      <  2:520665/80‑1 (MQ=255)                                                                         cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGaa    <  2:520667/80‑1 (MQ=255)                                                                         cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt   >  2:520718/1‑81 (MQ=255)                                                                         cATGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt   >  1:520737/1‑81 (MQ=255)                                                                          aTGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAAt   >  2:520726/1‑80 (MQ=255)                                                                          aTGAGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATc  <  1:520668/81‑1 (MQ=255)                                                                             |                                                                              AACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATC  >  NC_000913/2827590‑2827743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑