Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,832,654	T→A	V60E (GTG→GAG)	norV →	anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,832,654	0	T	A	100.0%	98.8 / NA	28	V60E (GTG→GAG)	norV	anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/10);  total (18/10)

CAATAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGTGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAC  >  NC_000913/2832580‑2832727                                                                           |                                                                          cAATAGCTACCTCATCCGCGAAGGAAAAAACGTGCTGATCGACACCGACGACCATAAATTCAGCCGCGAATTTGAGCAg                                                                       <  2:521837/79‑1 (MQ=255)  aaTAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAAc                                                                    >  2:521889/1‑81 (MQ=255)   aTAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAAcc                                                                   >  2:521876/1‑81 (MQ=255)    tAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCt                                                                  <  1:521838/81‑1 (MQ=255)     aGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTg                                                                 >  2:521888/1‑81 (MQ=255)      gCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGc                                                                <  2:521839/81‑1 (MQ=255)      gCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGc                                                                >  1:521873/1‑81 (MQ=255)         aCCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGt                                                              >  2:521867/1‑80 (MQ=255)         aCCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGt                                                              >  1:521870/1‑80 (MQ=255)           cTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAAt                                                           >  2:521869/1‑81 (MQ=255)                 cgcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAAt                                                      <  2:521840/80‑1 (MQ=255)                 cgcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATc                                                     >  2:521877/1‑81 (MQ=255)                  gcgAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATc                                                     >  1:521885/1‑80 (MQ=255)                    gaagaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGAt                                                  >  1:521887/1‑81 (MQ=255)                    gaagaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGAt                                                  <  2:521841/81‑1 (MQ=255)                      agaaAAAAACGCGCTGATCGACACCGTCGACCATAAATCCAGACGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCt                                                >  2:521883/1‑81 (MQ=255)                       gaaAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTg                                               <  1:521842/81‑1 (MQ=255)                        aaaaaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTgg                                              >  2:521884/1‑81 (MQ=255)                         aaaaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGc                                             <  2:521843/81‑1 (MQ=255)                           aaaaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCg                                            >  1:521868/1‑80 (MQ=255)                             aaCGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGat                                         <  2:521844/81‑1 (MQ=255)                                gTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATc                                      >  1:521896/1‑81 (MQ=255)                                           cacCGTCGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGt                           <  2:521845/81‑1 (MQ=255)                                                 cGACCATAAATTCAGCCGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTaa                     >  1:521875/1‑81 (MQ=255)                                                                ccGCGAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGa      >  1:521907/1‑81 (MQ=255)                                                                 cgcgAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGAc     >  1:521874/1‑81 (MQ=255)                                                                  gcgAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGAcc    <  2:521846/81‑1 (MQ=255)                                                                    gAATTTGAGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAc  >  2:521894/1‑81 (MQ=255)                                                                           |                                                                          CAATAGCTACCTCATCCGCGAAGAAAAAAACGTGCTGATCGACACCGTCGACCATAAATTCAGCCGCGAATTTGTGCAGAACCTGCGTAATGAAATCGATCTGGCGGATATCGATTACATCGTGATTAACCATGCAGAAGAGGACCAC  >  NC_000913/2832580‑2832727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑