Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,396	G→A	V119V (GTC→GTT)	hycF ←	formate hydrogenlyase subunit HycF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,396	0	G	A	100.0%	105.7 / NA	29	V119V (GTC→GTT)	hycF	formate hydrogenlyase subunit HycF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

GCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCT  >  NC_000913/2844324‑2844471                                                                         |                                                                            gCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCgg                                                                       >  1:524549/1‑79 (MQ=255)     tCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTg                                                                 <  1:524509/81‑1 (MQ=255)       gCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGca                                                               >  2:524551/1‑81 (MQ=255)       gCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGc                                                                <  2:524510/80‑1 (MQ=255)        ccGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGcac                                                              <  1:524511/81‑1 (MQ=255)                     gcagcGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCgg                                                  >  2:524547/1‑80 (MQ=255)                        gcgcAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACt                                              >  1:929865/1‑81 (MQ=255)                          gcAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                            <  1:524512/81‑1 (MQ=255)                          gcAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                            >  2:524553/1‑81 (MQ=255)                          gcAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                            <  2:524513/81‑1 (MQ=255)                            aaTGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTg                                          <  2:524514/81‑1 (MQ=255)                                gCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAgg                                      <  2:524516/81‑1 (MQ=255)                                   tAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAg                                   <  1:524517/81‑1 (MQ=255)                                    aGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGATTGTTGCAGGAAGt                                  >  2:524552/1‑81 (MQ=255)                                     gTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtc                                 <  1:524518/81‑1 (MQ=255)                                     gTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGt                                  <  2:524519/80‑1 (MQ=255)                                          tctcTTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTcttctt                            >  2:524561/1‑81 (MQ=255)                                             ctTTCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttct                         >  2:524565/1‑81 (MQ=255)                                              tttCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTcttctt                            <  1:524520/77‑1 (MQ=255)                                                tCTGGACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttct                         <  2:524521/78‑1 (MQ=255)                                                   ggACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCcaca                   >  2:524558/1‑81 (MQ=255)                                                   ggACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCcaca                   >  2:524557/1‑81 (MQ=255)                                                    gACGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCacac                  >  2:524563/1‑81 (MQ=255)                                                     aCGGCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACAcc                 >  1:524560/1‑81 (MQ=255)                                                        gCGAAAGGACGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGcc              <  2:524522/81‑1 (MQ=255)                                                                aCGATTGCAAACGCTGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTa      >  1:524564/1‑81 (MQ=255)                                                                aCGATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTa      >  2:524550/1‑81 (MQ=255)                                                                  gATTGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTAct    >  2:524574/1‑81 (MQ=255)                                                                    ttGCAAACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActct  <  1:524523/81‑1 (MQ=255)                                                                         |                                                                            GCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCT  >  NC_000913/2844324‑2844471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑