Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,308	T→C	F183F (TTT→TTC)	pphB →	phosphoprotein phosphatase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,308	0	T	C	100.0%	63.7 / NA	19	F183F (TTT→TTC)	pphB	phosphoprotein phosphatase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/6);  total (13/6)

TTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGC  >  NC_000913/2860234‑2860378                                                                           |                                                                       ttACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATAttt                                                                  <  2:528380/81‑1 (MQ=255)   aCTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTg                                                                 >  2:528403/1‑80 (MQ=255)    ctctGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGa                                                               >  1:528414/1‑81 (MQ=255)      ctGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACa                                                             >  1:528409/1‑81 (MQ=255)                   cGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGa                                                 >  1:528415/1‑80 (MQ=255)                     tgtgCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATaa                                              >  1:528407/1‑81 (MQ=255)                                    tAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc                               >  1:528420/1‑81 (MQ=255)                                    tAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc                               >  1:528408/1‑81 (MQ=255)                                     aaTGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc                               >  2:528416/1‑80 (MQ=255)                                      aTGGCGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCt                              >  2:528406/1‑80 (MQ=255)                                          cGAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAAc                           >  1:528417/1‑79 (MQ=255)                                           gAGTTACAACAAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAAc                           >  2:528381/1‑78 (MQ=255)                                                acaacaAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACTAGATTta                   >  2:528413/1‑81 (MQ=255)                                                acaacaAATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAttt                    <  2:528382/80‑1 (MQ=255)                                                    caaATAAACGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATAtt               <  2:528383/81‑1 (MQ=255)                                                           aCGGCGCTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCg        <  2:528384/81‑1 (MQ=255)                                                               cgcTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc    <  2:528385/81‑1 (MQ=255)                                                               cgcTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc    <  1:528386/81‑1 (MQ=255)                                                                 cTGATTATTTCATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGc  >  1:528411/1‑81 (MQ=255)                                                                           |                                                                       TTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGC  >  NC_000913/2860234‑2860378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑